高效液相色谱法同时测定水辣蓼提取物中6种黄酮成分的含量
建立了同时定量检测水辣蓼中6种黄酮类成分的高效液相色谱方法：选择Waters Symmetry shield RP18（4.6 mm×250 mm,5.0 μm）色谱柱,流动相甲醇-0.4％甲酸水溶液（梯度洗脱）,流速1 mL/min,柱温30℃,检测波长359 nm,进样量10 μL.结果表明,6种黄酮成分在50 min内达到基线分离,在0.1～20μg/mL浓度范围内,6种黄酮成分的线性相关系数R2≥0.9995,平均加样回收率为96.65％～99.54％,RSD为0.82％～ 1.38％ （n=3）.该方法准确、简便,重复性好,可用于水辣蓼中芦丁、金丝桃苷、槲皮苷、槲皮素、异鼠李素和山奈...
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| Published in | 广东农业科学 Vol. 41; no. 13; pp. 94 - 98 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
广东药学院中药学院,广东广州,510006%广东省农科院动物卫生研究所,广东广州,510640
2014
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1004-874X |
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| Summary: | 建立了同时定量检测水辣蓼中6种黄酮类成分的高效液相色谱方法：选择Waters Symmetry shield RP18（4.6 mm×250 mm,5.0 μm）色谱柱,流动相甲醇-0.4％甲酸水溶液（梯度洗脱）,流速1 mL/min,柱温30℃,检测波长359 nm,进样量10 μL.结果表明,6种黄酮成分在50 min内达到基线分离,在0.1～20μg/mL浓度范围内,6种黄酮成分的线性相关系数R2≥0.9995,平均加样回收率为96.65％～99.54％,RSD为0.82％～ 1.38％ （n=3）.该方法准确、简便,重复性好,可用于水辣蓼中芦丁、金丝桃苷、槲皮苷、槲皮素、异鼠李素和山奈酚的含量测定,为控制水辣蓼药材的质量提供依据. |
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| Bibliography: | Polygonum hydropiper L. ; flavonoid; HPLC; content determination 44-1267/S HE Li-mei, CHEN Yu-ting, LIU Hong-mei, PENG Xin-yu, PAN Yu-fang, YUAN Ming-gui,LUO Sheng-jun,WEI Guang-wei （ 1. College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China; 2. Institute of A nimal Health, Guangdong Academy of Agricaltural Sciences, Guangzhou 510640, China） This paper established a method of quantitative detection for six kinds of flavonoid content in extract of Polygonum hydropiper L. by high performance liquid chromatography （HPLC）. The analysis was performed on a Waters Symmetry shield RP18 （4.6 mm×250 mm, 5.0μm） column. Mobile phase was methanol-0.4% formic acid aqueous solution （gradient elution）. The flow rate was set at 1.0 mL/min and the eluent was detected at 359 nm at 30℃. The calibration curves of the six kinds of flavonoid were linear（R2〉0.9995） over the concentration range of 0.1-20 μg/mL. The mean recoveries were 96.65% to 99.54%, RSD was 0.82%-1.38% （n=3）. The result |
| ISSN: | 1004-874X |