油茶根际溶磷菌3-Y-08的筛选与鉴定

采用传统的微生物分离培养法,对油茶根际溶磷菌进行分离,筛选出6株溶磷细菌。利用透明圈法对油茶根际土壤中具有溶磷能力的细菌进行初筛;采用钼锑抗比色法测定发酵液的可溶性磷含量,对解磷菌株进行复筛,得出菌株3-Y-08的溶磷活性最强。根据进行菌落形态特征、生理生化特征、16S rDNA序列和系统发育分析等研究,初步鉴定菌株3-Y-08为巨大芽孢杆菌。...

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Published in广东农业科学 Vol. 42; no. 3; pp. 47 - 50
Main Author 刘小玉 付登强 陈良秋 杨伟波 李东霞 符海泉 贾效成
Format Journal Article
LanguageChinese
Published 中国热带农业科学院椰子研究所/油茶研究中心,海南文昌,571339 2015
Subjects
油茶
溶磷菌
筛选
鉴定
油茶
筛选
鉴定
溶磷菌
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ISSN1004-874X

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Summary:采用传统的微生物分离培养法,对油茶根际溶磷菌进行分离,筛选出6株溶磷细菌。利用透明圈法对油茶根际土壤中具有溶磷能力的细菌进行初筛;采用钼锑抗比色法测定发酵液的可溶性磷含量,对解磷菌株进行复筛,得出菌株3-Y-08的溶磷活性最强。根据进行菌落形态特征、生理生化特征、16S rDNA序列和系统发育分析等研究,初步鉴定菌株3-Y-08为巨大芽孢杆菌。
Bibliography:44-1267/S
LIU Xiao-yu, FU Deng-qiang, CHEN Liang-qiu, YANG Wei-bo, LI Dong-xia, FU Hai-quan,JIA Xiao-cheng （Coconut Research Institute/Oil Camellia Research Center, CA TAS, Wenchang 571339, China）
camellia; phosphate solubilizing bacteria; screening; identification
Phosphate solubilizing bacteria were isolated from the rhizosphere of camellia,and six strains were screened by using traditional method of isolation and culture of microorganisms. By using the way of clear zone, phosphate solubilizing bacteria from the rhizosphere soil were preliminarily isolated, and the high effective phosphate solubilizing bacteria were further screened by using molybdenum-antimony anti-spectrophotometric method, strain 3- Y-08 was the most active. The strains and 16S rDNA and phylogenetic tree were identified by studying the morphology, cultural, physiological and biochemical properties. Thus the strain was preliminarily identified as Bacillus megaterium.
ISSN:1004-874X