海南生姜青枯病菌对农用链霉素的敏感性测定

从海南白沙、文昌等市县生姜主要种植区采样,经分离、纯化,获得生姜青枯病菌（Ralstonia solanacearum）菌株27株,采用抑菌圈法对72%农用链霉素可湿性粉剂进行了敏感性测定。结果表明：不同地理来源的生姜青枯病菌对农用链霉素敏感性差异明显,同一来源地的不同菌株也有一定的差异。EC50值分布在3.22-71.07μg/m L,平均EC50为34.69μg/m L;其中,白沙荣邦RB-2菌株最敏感,EC50为3.22μg/m L;而白沙邦溪BX-3菌株敏感性最低,EC50为71.07μg/m L。敏感性分布频率呈连续性单峰曲线,EC50值表现连续,符合正态分布,其平均EC50值34....

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Bibliographic Details
Published in西南农业学报 Vol. 29; no. 3; pp. 570 - 573
Main Author 赵志祥 严婉荣 陈圆 陈绵才 肖敏 肖彤斌
Format Journal Article
LanguageChinese
Published 海南省农业科学院农业环境与植物保护研究所,海南省植物病虫害防控重点实验室,海南海口571100 2016
Subjects
EC50值
农用链霉素
敏感性
敏感性基线
生姜青枯病菌
农用链霉素
Sensitivity
Streptomycin
Ralstonia solanacearum
敏感性基线
EC50值
Sensitivity baseline
生姜青枯病菌
敏感性
EC50 value
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2016.03.020

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Summary:从海南白沙、文昌等市县生姜主要种植区采样,经分离、纯化,获得生姜青枯病菌（Ralstonia solanacearum）菌株27株,采用抑菌圈法对72%农用链霉素可湿性粉剂进行了敏感性测定。结果表明：不同地理来源的生姜青枯病菌对农用链霉素敏感性差异明显,同一来源地的不同菌株也有一定的差异。EC50值分布在3.22-71.07μg/m L,平均EC50为34.69μg/m L;其中,白沙荣邦RB-2菌株最敏感,EC50为3.22μg/m L;而白沙邦溪BX-3菌株敏感性最低,EC50为71.07μg/m L。敏感性分布频率呈连续性单峰曲线,EC50值表现连续,符合正态分布,其平均EC50值34.69μg/m L可作为海南生姜青枯病菌对农用链霉素的敏感性基线,用于海南生姜青枯病菌对农用链霉素的抗药性监测。
Bibliography:Ralstonia solanacearum; Streptomycin ; EC50 value; Sensitivity; Sensitivity baseline
51-1213/S
Samples were collected from ginger regions in Baisha and Wenchang of Hainan. After isolation and purification, twenty-seven strains of Ralstonia solanacearum were obtained in our laboratory. Then bactericide sensitivity to 72 % streptomycin of these strains were determined using the method of inhibition zone. The results showed that there were sensitivity differences among different geographical origin. And different strains from the same sources also had certain difference. EC50 value ranged from 3.22 -71.07μ g/mL,with the average value being 34.69 μg/mL. Strain RB-2 from Rongbang of Balsha was the most sensitive to the bactericide,with the EG50 value of 3.22 μg/mL. But BX-3 from Bangxi of Baisha was strains of the lowest sensitivity, with the EC50 value of 71.07 μg/mL. The frequency distribution of sensitivity showed that the EC50 value displayed succession and fit the normal distribution. The sensitivity baseline o
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2016.03.020