冷处理后水稻苗期差异蛋白的表达与分析
水稻ZH-ct为ZH11在组织培养过程中发现一株突变单株,具有较好的苗期耐冷性,通过人工气候箱低温处理,应用蛋白质双向凝胶电泳（2-DE）检测其低温处理后的差异蛋白表达,并对进行分析。结果显示,共发现差异蛋白468个,对其中重复性好,表达量较高的14个蛋白进行质谱分析,除2个没有检测到蛋白信号外,其余12个分别为光合作用、能量代谢、渗透压调节、过氧化物酶等功能蛋白,为下一步进行耐冷的分子基础及反向遗传学分析提供了基础。...
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| Published in | 西南农业学报 Vol. 29; no. 3; pp. 520 - 524 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
宜春学院生命科学与资源环境学院,江西宜春336000
2016
云南农业大学农学与生物技术学院,云南昆明650201%云南农业大学农学与生物技术学院,云南昆明,650201%华南农业大学生命科学学院,广东广州,510642%国家杂交水稻改良中心高原分中心,云南个旧,661000 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
| DOI | 10.16213/j.cnki.scjas.2016.03.010 |
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| Summary: | 水稻ZH-ct为ZH11在组织培养过程中发现一株突变单株,具有较好的苗期耐冷性,通过人工气候箱低温处理,应用蛋白质双向凝胶电泳（2-DE）检测其低温处理后的差异蛋白表达,并对进行分析。结果显示,共发现差异蛋白468个,对其中重复性好,表达量较高的14个蛋白进行质谱分析,除2个没有检测到蛋白信号外,其余12个分别为光合作用、能量代谢、渗透压调节、过氧化物酶等功能蛋白,为下一步进行耐冷的分子基础及反向遗传学分析提供了基础。 |
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| Bibliography: | ZHOU Guo-hua, LIU Tao, ZHANG Xue-mei , ZHANG Qun-yu , LI Xiao-fen （1. College of Life Sciences and Environment Resource, Yichun University, Jiangxi Yichun 336000, China; 2. College of Agronomy and Bio-technology, Yunnan Agricultural University, Yunnan Kunming 650201, China; 3. College of Life Science, South China Agricultural University, Guangdong Guangzhou 510642, China; 4. Plateau Division of China National Hybrid Rice Center, Yunnan Gejiu 661000, China） ZH-ct possessed the characters of good cold tolerance, which was a mutation plants from tissue culture of zhl 1 callus. Its differential protein expressions in the artificial climate chamber after low temperature treatment by 2-DE were studied. The result showed that there were 468 differential expression proteins spots signed by it＇ s relative software, in which 14 spots had a better replication and were easily to slice, which were further analyzed by mass spectra, and all of them, except 2 spots were no signs, were photosynthesis, supersession, os- motic p |
| ISSN: | 1001-4829 |
| DOI: | 10.16213/j.cnki.scjas.2016.03.010 |