新西兰大白兔组织工程骨的构建及体内异位成骨效果

目的：探讨新西兰大白兔组织工程骨股骨旁异位成骨（ectopic bone formation）的效果.方法：选取新西兰大白兔骨髓基质干细胞进行成骨诱导,与生物陶瓷构建组织工程骨,种植到新西兰大白兔股骨的骨膜旁,第8周获得异位成骨组织,检测分析收获的异位成骨材料结构.结果：第8周的异位成骨材料内有骨组织形成,成骨材料结构清晰,组织内部有新血管形成,荧光蛋白标记的标本有荧光蛋白表达.结论：组织工程骨在体内可顺利异位成骨....

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Bibliographic Details
Published in中国临床医学 Vol. 24; no. 1; pp. 89 - 92
Main Author 李海旭 石继祥 石文俊 刘孚瑛 章筛林 周强
Format Journal Article
LanguageChinese
Published 上海中医药大学附属普陀医院骨科,上海,200062 2017
Subjects
异位成骨
组织工程骨
结构
engineered bone constructs
组织工程骨
异位成骨
结构
structure
ectopic bone formation
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ISSN1008-6358
DOI10.12025/j.issn.1008-6358.2017.20160775

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Summary:目的：探讨新西兰大白兔组织工程骨股骨旁异位成骨（ectopic bone formation）的效果.方法：选取新西兰大白兔骨髓基质干细胞进行成骨诱导,与生物陶瓷构建组织工程骨,种植到新西兰大白兔股骨的骨膜旁,第8周获得异位成骨组织,检测分析收获的异位成骨材料结构.结果：第8周的异位成骨材料内有骨组织形成,成骨材料结构清晰,组织内部有新血管形成,荧光蛋白标记的标本有荧光蛋白表达.结论：组织工程骨在体内可顺利异位成骨.
Bibliography:31-1794/R
engineered bone constructs;ectopic bone formation;structure
Objective：To study the structure of ectopic bone formed beside the New-Zealand rabbit femoral bone. Methods：Bone marrow stroma stem cells （BMSCs）were taken from New-Zealand rabbits to built engineered bone constructs, the constructs were embedded near the rabbit femoral bone,the ectopic bone constructs were harvested at 8^th week for further examination and study.Results：At 8th week,the ectopic bone had a clear bone-like structure,together with new blood supply, green fluorescent protein could be seen in samples seeded by marked cells.Conclusions：This study showed that the ectopic bone formation could be successfulinvivo.
LI Hai-xu, SHI Ji-xiang, SHI Wen-jun, LIU Fu-ying, ZHANG Shai-lin, ZHOU Qiang（Department of Orthopaedic, Putuo Hospitals Shanghai Traditional Medical University, Shanghai 200062, China）
ISSN:1008-6358
DOI:10.12025/j.issn.1008-6358.2017.20160775