马铃薯引进品种及变异材料的SSR遗传多样性分析

为了鉴定马铃薯引进品种和变异材料的遗传背景和亲缘关系,利用9对SSR引物对12个引进品种、12个费乌瑞它自然和辐射变异材料进行遗传多样性分析.结果表明：对24份马铃薯参试材料的DNA进行PCR扩增共获得178条扩增条带,其中167个为多态性条带,多态性比率为93.8％,平均每对引物扩增条带数为18.6条.多态性条带中有84.4％的条带大小为120 ～ 950bp.24份参试材料的遗传相似性系数在0.479 ～1.000.聚类分析显示,在遗传相似系数为0.65处,参试材料可明显分为4个类群,全部变异材料均聚在1个类群,品种间的多态性和遗传距离大于变异材料....

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Bibliographic Details
Published in西南农业学报 Vol. 27; no. 4; pp. 1399 - 1403
Main Author 黄先群 冉江 黄团 李丽
Format Journal Article
LanguageChinese
Published 贵州省农业科学院重点实验室/贵州省生物技术研究所,贵州贵阳,550006%贵州省农业科学院重点实验室/贵州省生物技术研究所,贵州贵阳550006 2014
贵州大学生命科学学院,贵州贵阳550025
Subjects
SSR标记
变异
引进品种
遗传多样性
马铃薯
变异
Potato
遗传多样性
SSR markers
Introduced variety
Genetic diversity
Mutation
引进品种
马铃薯
SSR标记
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ISSN1001-4829

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Summary:为了鉴定马铃薯引进品种和变异材料的遗传背景和亲缘关系,利用9对SSR引物对12个引进品种、12个费乌瑞它自然和辐射变异材料进行遗传多样性分析.结果表明：对24份马铃薯参试材料的DNA进行PCR扩增共获得178条扩增条带,其中167个为多态性条带,多态性比率为93.8％,平均每对引物扩增条带数为18.6条.多态性条带中有84.4％的条带大小为120 ～ 950bp.24份参试材料的遗传相似性系数在0.479 ～1.000.聚类分析显示,在遗传相似系数为0.65处,参试材料可明显分为4个类群,全部变异材料均聚在1个类群,品种间的多态性和遗传距离大于变异材料.
Bibliography:HUANG Xian-qun , RAN Jiang , HUANG Tuan , LI Li （ 1. Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Institute of Biotechnology, Guizhou Guiyang 550006, China; 2. College of Life Sciences, Guizhou University, Guizhou Guiyang 550025, China）
Potato; Introduced variety; Mutation; SSR markers; Genetic diversity
51-1213/S
In order to identify the genetic background and relationship of the introduced varieties and mutation materials in potato, the genetic diversity of 12 introduced varieties and 12 natural and radial mutation materials of potato variety ‘ Favorita’ were analyzed by 9 pairs SSR markers. Results： There were 178 amplified bands, 167 polymorphism bands of which were obtained with a polymorphism rate of 93.8 %. The average of each SSR primer amplified was 18.6 bands. There were 84.4 % of polymorphism bands located in 120 -950 bp. The genetic similarity coe~cients of the 24 experimental materials were from 0. 479 to 1. 000. The UPGMA cluster analysis showed that all experimental materials coul
ISSN:1001-4829