谢瓦氏曲霉间型变种veA基因全长克隆及序列分析

为进一步研究谢瓦氏曲霉间型变种中veA基因的结构及其与有性发育的关系,以谢瓦氏曲霉间型变种为有性发育研究材料对veA基因进行全长克隆,根据已克隆到的veA保守区片段设计基因特异性引物（GSP）,使用RACE技术从总RNA克隆到了长度为2 515 bp的veA基因的cDNA。序列分析表明,该cDNA编码有562个氨基酸残基,其预测蛋白质序列与丝状真菌的其他种相应预测蛋白质比对结果发现,其相似性较好,保守区序列集中于蛋白质的N端。进一步研究表明,该基因开放阅读框内只含一个内含子,长度为54 bp,位于起始密码子下游149 bp处。说明,已成功地从谢瓦氏曲霉间型变种中克隆到了veA基因。...

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Bibliographic Details
Published in西南农业学报 Vol. 25; no. 1; pp. 136 - 139
Main Author 马权 刘永翔 刘作易
Format Journal Article
LanguageChinese
Published 贵州省农业科学院,贵州贵阳550006 2012
铜仁职业技术学院医学系,贵州铜仁554300
贵州省农业生物技术重点实验室,贵州贵阳550006%贵州省农业生物技术重点实验室,贵州贵阳550006
贵州省生物技术研究所,贵州贵阳550006%贵州省农业生物技术重点实验室,贵州贵阳550006
Subjects
veA基因
全长克隆
有性发育
谢瓦氏曲霉间型变种
veA基因
有性发育
全长克隆
谢瓦氏曲霉间型变种
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ISSN1001-4829
DOI10.3969/j.issn.1001-4829.2012.01.029

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Summary:为进一步研究谢瓦氏曲霉间型变种中veA基因的结构及其与有性发育的关系,以谢瓦氏曲霉间型变种为有性发育研究材料对veA基因进行全长克隆,根据已克隆到的veA保守区片段设计基因特异性引物（GSP）,使用RACE技术从总RNA克隆到了长度为2 515 bp的veA基因的cDNA。序列分析表明,该cDNA编码有562个氨基酸残基,其预测蛋白质序列与丝状真菌的其他种相应预测蛋白质比对结果发现,其相似性较好,保守区序列集中于蛋白质的N端。进一步研究表明,该基因开放阅读框内只含一个内含子,长度为54 bp,位于起始密码子下游149 bp处。说明,已成功地从谢瓦氏曲霉间型变种中克隆到了veA基因。
Bibliography:51-1213/S
Aspergillus chevalieri var.intermedium; veA gene; Sexual development; Full-length cloning
The full-length of veA gene from A.chevalieri var.intermedium was cloned to further explore the structure and sexual development.The gene-specific primer（GSP） was designed according to the cloned veA conserved domains.And cDNA of the veA with length of 2 515 bp was cloned from total RNA by using RACE technique.Sequence analysis showed that the cDNA encoded 562 amino acid residues.The predicted protein has sequence similarity with that in other species of filamentous fungi,and the conserved domain is located at the N-terminal of the protein.This study showed that open reading frame of the gene only contained one intron located at downstream 149 bp of the start codon with length of 54 bp.This result showed the veA gene had been cloned from the A.chevalieri var.intermedium successfully.
MA Quan,LIU Yong-xiang,LIU Zuo-yi（1.Department of Medicine,Tongren Vocational and Technical College,Guizhou Tongren 554300,China;2.G
ISSN:1001-4829
DOI:10.3969/j.issn.1001-4829.2012.01.029