巨尾桉组培快繁与转基因再生体系的优化

以巨尾桉带腋芽的半木质化茎段为最初外植体．通过正交试验优化法，对巨尾桉组培快繁体系建立过程中各项培养基进行了研究，建立起良好的巨尾桉组织培养再生体系，为桉树遗传转化和良种保存提供材料和技术支持，同时对根癌农杆菌介导的叶盘法转化体系进行优化。结果表明：叶片分化芽率提高到26．7％，理论上最佳的转基因巨尾桉叶片直接分化培养基为WPM＋6-BA0．3mg／L＋NAA0．15mg／L，生根培养基中筛选转基因芽段的卡那霉素选择压为20mg／L。...

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Bibliographic Details
Published in广东农业科学 Vol. 39; no. 22; pp. 51 - 54
Main Author 周利建 赵艳玲
Format Journal Article
LanguageChinese
Published 华侨大学化工学院,福建厦门,361021 2012
Subjects
巨尾桉
正交设计
组织培养
转基因
组织培养
正交设计
转基因
巨尾桉
Online AccessGet full text
ISSN1004-874X

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Summary:以巨尾桉带腋芽的半木质化茎段为最初外植体．通过正交试验优化法，对巨尾桉组培快繁体系建立过程中各项培养基进行了研究，建立起良好的巨尾桉组织培养再生体系，为桉树遗传转化和良种保存提供材料和技术支持，同时对根癌农杆菌介导的叶盘法转化体系进行优化。结果表明：叶片分化芽率提高到26．7％，理论上最佳的转基因巨尾桉叶片直接分化培养基为WPM＋6-BA0．3mg／L＋NAA0．15mg／L，生根培养基中筛选转基因芽段的卡那霉素选择压为20mg／L。
Bibliography:ZHOU Li-jian, ZHAO Yan-ling （College of Chemical Engineering, Huaqiao University, Xiamen 361021, China）
44-1267/S
Eucalyptus grandisxE, ophylla; tissue culture; orthogonal design; genetic transformation
Efficient tissue culture regeneration system of Eucalyptus grandis xE. ophylla was established by orthogonal optimization method. The stem-segments with axillary buds from aseptic seedlings were used as exp[ants to reproduce multiple buds. The system of agrobacterium-mediated transformation of Eucalyptus was optimized and the differentiation rate increased to 26.7%. Theoretically, the most suitable medium for direct differentiation of adventitious buds was WPM＋6-BA 0.3 mg/L＋NAA 0.15 mg/L and the Km resistance level of the transgenic plants was 20 mg/L.
ISSN:1004-874X