子宫内膜异位症患者腹腔液单个核细胞IFN-γ及IL-10mRNA的表达及意义

目的探讨子宫内膜异位症（EM）患者腹腔液单个核细胞（PFMC）γ干扰素（IFN-γ）及白细胞介素（IL）-10mRNA的表达水平及其意义。方法选取经手术后病理证实为EM的患者55例作为EM组，以非EM患者52例作为对照组。分离PFMC，应用TaqMan实时荧光逆转录聚合酶链反应（RT-PCR）测定IFN-γ及IL-10mRNA的表达水平。结果EM组PFMCIFN-γmRNA的表达水平低于对照组，而IL-10mRNA的表达水平明显高于对照组（P<0.05），EM组IFN-γ/IL-10比值较对照组明显降低（P<0.05）；Ⅰ~Ⅱ期EM患者IFN-γ/IL-10比值高于...

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Bibliographic Details
Published in天津医药 Vol. 45; no. 7; pp. 745 - 747
Main Author 李津鸿;王玉亮
Format Journal Article
LanguageChinese
Published 天津医科大学总医院医学检验科 300052%天津市第一中心医院医学检验科 2017
Subjects
子宫内膜异位症;干扰素Ⅱ型;白细胞介素10;单个核细胞;腹腔液
单个核细胞
mononuclear cells
子宫内膜异位症
白细胞介素10
腹腔液
interferon type
endometriosis
peritoneal fluid
干扰素Ⅱ型
interleukin 10
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ISSN0253-9896
DOI10.11958/20170353

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Summary:目的探讨子宫内膜异位症（EM）患者腹腔液单个核细胞（PFMC）γ干扰素（IFN-γ）及白细胞介素（IL）-10mRNA的表达水平及其意义。方法选取经手术后病理证实为EM的患者55例作为EM组，以非EM患者52例作为对照组。分离PFMC，应用TaqMan实时荧光逆转录聚合酶链反应（RT-PCR）测定IFN-γ及IL-10mRNA的表达水平。结果EM组PFMCIFN-γmRNA的表达水平低于对照组，而IL-10mRNA的表达水平明显高于对照组（P<0.05），EM组IFN-γ/IL-10比值较对照组明显降低（P<0.05）；Ⅰ~Ⅱ期EM患者IFN-γ/IL-10比值高于Ⅲ~Ⅳ期患者，差异有统计学意义（P<0.05）。结论EM患者PFMC中IFN-γ/IL-10失衡可能与EM的发生发展有关。
Bibliography:endometriosis; interferon type Ⅱ; interleukin 10; mononuclear cells; peritoneal fluid
Objective To investigate the expression levels and significance of interferon-γ(IFN-γ),interleukin(IL)-10mRNA in peritoneal fluid mononuclear cells(PFMC)in patients with endometriosis(EM).Methods A total of55patients with EM diagnosed histopathologically after operation were enrolled as EM group,and52patients without EM were enrolled as control group.The PFMC were isolated,and the expression levels of IFN-γand IL-10mRNA were determined by TaqMan real-time reverse transcriptase-polymerase chain reaction(RT-PCR).Results It was found that IFN-γmRNA expression level was significantly decreased in EM group compared with that in control group(P<0.05),while IL-10mRNA expression level was significantly increased in EM group compared with that in control group(P<0.05).The ratio of IFN-γ/IL-10was significantly lower in EM group than that in control group(P<0.05).The ratio of IFN-γ/IL-10was significantly higher in patients with
ISSN:0253-9896
DOI:10.11958/20170353