不同地理群体的鲇线粒体DNA 16S rRNA基因序列比较分析

对长江中上游主要支流5个野生群体鲇〔舞阳河群体（WYH）、乌江群体（WJ）、雅砻江群体（YLJ）、岷江群体（MJ）、金沙江群体（CS）〕中的27个样品的16S rRNA基因进行PCR扩增,并对扩增产物测序。用CLUSTAL X v 1.81软件对所得的27个16SrRNA序列进行比对,并用Dna SP 5.10软件进行比较分析,共检测出74个变异位点、8种单倍型。舞阳河、乌江、雅砻江、岷江、金沙江等5个野生群体的单倍型多样性（H）分别为0.600、0.857、0.600、0.600、0.667,核苷酸多样性（π）分别为0.00072、0.1754、0.00036、0.00072、0.01871...

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Bibliographic Details
Published in广东农业科学 Vol. 40; no. 1; pp. 150 - 153
Main Author 王庆容 宋培勇 唐立俊 喻哓丹
Format Journal Article
LanguageChinese
Published 遵义师范学院生物系,贵州遵义,563002 2013
Subjects
16SrRNA基因
线粒体DNA
遗传多样性
鲇
线粒体DNA
16S rRNA基因
遗传多样性
鲇
Online AccessGet full text
ISSN1004-874X

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Summary:对长江中上游主要支流5个野生群体鲇〔舞阳河群体（WYH）、乌江群体（WJ）、雅砻江群体（YLJ）、岷江群体（MJ）、金沙江群体（CS）〕中的27个样品的16S rRNA基因进行PCR扩增,并对扩增产物测序。用CLUSTAL X v 1.81软件对所得的27个16SrRNA序列进行比对,并用Dna SP 5.10软件进行比较分析,共检测出74个变异位点、8种单倍型。舞阳河、乌江、雅砻江、岷江、金沙江等5个野生群体的单倍型多样性（H）分别为0.600、0.857、0.600、0.600、0.667,核苷酸多样性（π）分别为0.00072、0.1754、0.00036、0.00072、0.01871。对5个野生群体的16S rRNA序列进行Tajima’s D和Fst分析发现,所有群体符合中性进化模型,且有一些单倍型的分化,只有金沙江群体的遗传差异显著。对5个群体进行分子变异等级分析的结果表明,群体间分子变异不显著。对5个群体构建分子系统树发现,乌江、雅砻江、岷江、金沙江群体之间的亲缘关系较近,舞阳河群体与其他群体间的亲缘关系较远。
Bibliography:44-1267/S
WANG Qing-rong,SONG Pei-yong,TANG Li-jun,YU Xiao-dan（Department of Biology,Zunyi Normal College,Zunyi 563002,China）
Silurus asotus; mitochondrial DNA; genetic diversity; 16S rRNA gene
The aim of the present study was to survey mtDNA 16S rRNA sequence variation to assess genetic diversity and genetic structure of the wild Silurus asotus in the upstream and midstream of Yangtze River. The samples of S. asotus have been obtained from the midstream and upstream of Yangtze River included the Wuyanghe （WYH）, Wujiang River （WJ）, Yalong Jiang （YLJ）, Min River （MJ） and Chin-sha River （CS）. The PCR technique was used to amplify the mtDNA 16S rRNA among 27 individuals. The PCR products were purified and sequenced. As a result, 1 680 bp nueleotide sequences of 16S rRNA were obtained. 8 haplotypes and 74 variable sites were detected among all mitoehondrial 16S rRNA sequences of 27 Chinese catfish. Haplotype diversities （H） in the Wuyanghe putative population, Wujiang River putative population, Yalong Jiaug putative
ISSN:1004-874X