致病菌与非致病菌诱导对家蚕抗菌肽基因defensinA/B的表达定量分析
在致病菌（Bacillus bombyseptieus）与非致病菌（Escherichia coli）诱导家蚕后的不同时间,采用实时荧光定量PCR方法,对抗菌肽基因defensinA/B在脂肪体中的表达进行了定量分析。结果表明,在家蚕被注射黑胸败血芽孢杆菌致病菌和非致病菌（大肠杆菌）后的3、9、12 h,检测到脂肪体中的Bm-defA/B的转录活性上调,Bm-defB的响应活性高于Bm-defA,Bm-defB对黑胸败血芽孢杆菌致病菌的响应快于Bm-defA,提示Bm-defensins在家蚕对抗细菌侵染的免疫反应中发挥重要的作用。...
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| Published in | 西南农业学报 Vol. 26; no. 3; pp. 1282 - 1285 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101
2013
华南农业大学动物科学学院,广东广州510642%华南农业大学动物科学学院,广东广州,510642%云南省农业科学院蚕桑蜜蜂研究所,云南蒙自,661101 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
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| Abstract | 在致病菌（Bacillus bombyseptieus）与非致病菌（Escherichia coli）诱导家蚕后的不同时间,采用实时荧光定量PCR方法,对抗菌肽基因defensinA/B在脂肪体中的表达进行了定量分析。结果表明,在家蚕被注射黑胸败血芽孢杆菌致病菌和非致病菌（大肠杆菌）后的3、9、12 h,检测到脂肪体中的Bm-defA/B的转录活性上调,Bm-defB的响应活性高于Bm-defA,Bm-defB对黑胸败血芽孢杆菌致病菌的响应快于Bm-defA,提示Bm-defensins在家蚕对抗细菌侵染的免疫反应中发挥重要的作用。 |
|---|---|
| AbstractList | S851.34+7.31; 在致病菌(Bacilus bombyseptieus)与非致病菌(Escherichia coli)诱导家蚕后的不同时间,采用实时荧光定量PCR方法,对抗菌肽基因defensinA/B在脂肪体中的表达进行了定量分析.结果表明,在家蚕被注射黑胸败血芽孢杆菌致病菌和非致病菌(大肠杆菌)后的3、9、12 h,检测到脂肪体中Bm-defA/B的转录活性上调,Bm-defB的响应活性高于Bm-defA,Bm-defB对黑胸败血芽孢杆菌致病菌的响应快于Bm-defA,提示Bm-defensins在家蚕对抗细菌侵染的免疫反应中发挥重要的作用. 在致病菌（Bacillus bombyseptieus）与非致病菌（Escherichia coli）诱导家蚕后的不同时间,采用实时荧光定量PCR方法,对抗菌肽基因defensinA/B在脂肪体中的表达进行了定量分析。结果表明,在家蚕被注射黑胸败血芽孢杆菌致病菌和非致病菌（大肠杆菌）后的3、9、12 h,检测到脂肪体中的Bm-defA/B的转录活性上调,Bm-defB的响应活性高于Bm-defA,Bm-defB对黑胸败血芽孢杆菌致病菌的响应快于Bm-defA,提示Bm-defensins在家蚕对抗细菌侵染的免疫反应中发挥重要的作用。 |
| Author | 唐芬芬 杨伟克 邵榆岚 杨海 白兴荣 |
| AuthorAffiliation | 云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101 华南农业大学动物科学学院,广东广州510642 |
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| Author_FL | TANG Fen-fen YANG Wei-ke BAI Xing-rong SHAO Yu-lan YANG Hai |
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| DocumentTitleAlternate | Expression Quantitative Analysis of Bombyx mori Antimicrobial Peptides Genes Bm-defensinA/B Induced by Pthogens and Non-pathogens |
| DocumentTitle_FL | Expression Quantitative Analysis of Bombyx mori Antimicrobial Peptides Genes Bm-defensinA/B Induced by Pthogens and Non-pathogens |
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| Keywords | Real-time fluorescent quantitative PCR 家蚕 黑胸败血芽孢杆菌 defensinA/B 实时荧光定量PCR Escherichia coli 大肠杆菌 Bombyx mori Bacillus bombyseptieus |
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| Notes | In this paper,the real-time fluorescent quantitative PCR was applied to analyze the expression of Bm-defensinA/B in fat body after being injected pathogens（Bacillus bombyseptieus）and non-pathogens（Escherichia coli） of Bombyx mori.The results showed that defensinA and defensinB were all up-regulated after injection with B.bombyseptieus and E.coli at 3,9 and 12 h.Moreover,the expression of defensinB was higher and faster than that of defensinA.These suggested that Bm-defensins played an important role in B.mori immune response to against infection by pathogen. TANG Fen-fen1,2,YANG Wei-ke2,SHAO Yu-lan1,YANG Hai1,BAI Xing-rong1（1.Institute of Sericulture and Apiculture,Yunnan Academy of Agricultural Sciences,Yunnan Mengzi 661101,China;2.College of Animal Science,South China Agricultural University,Guangdong Guangzhou 510642,China） Bombyx mori ; Bacillus bombyseptieus ; Escherichia coil; DefensinA/ B ; Real-time fluorescent quantitative PCR 51-1213/S |
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| PublicationTitle | 西南农业学报 |
| PublicationTitleAlternate | Southwest China Journal of Agricultural Sciences |
| PublicationTitle_FL | Southwest China Journal of Agricultural Sciences |
| PublicationYear | 2013 |
| Publisher | 云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101 华南农业大学动物科学学院,广东广州510642%华南农业大学动物科学学院,广东广州,510642%云南省农业科学院蚕桑蜜蜂研究所,云南蒙自,661101 |
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| Snippet | 在致病菌（Bacillus bombyseptieus）与非致病菌（Escherichia coli）诱导家蚕后的不同时间,采用实时荧光定量PCR方法,对抗菌肽基因defensinA/B在脂肪体中的表达进行了定量... S851.34+7.31; 在致病菌(Bacilus bombyseptieus)与非致病菌(Escherichia coli)诱导家蚕后的不同时间,采用实时荧光定量PCR方法,对抗菌肽基因defensinA/B在脂肪体中的表达进... |
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| SubjectTerms | B defensinA 大肠杆菌 实时荧光定量PCR 家蚕 黑胸败血芽孢杆菌 |
| Title | 致病菌与非致病菌诱导对家蚕抗菌肽基因defensinA/B的表达定量分析 |
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