致病菌与非致病菌诱导对家蚕抗菌肽基因defensinA/B的表达定量分析

在致病菌（Bacillus bombyseptieus）与非致病菌（Escherichia coli）诱导家蚕后的不同时间,采用实时荧光定量PCR方法,对抗菌肽基因defensinA/B在脂肪体中的表达进行了定量分析。结果表明,在家蚕被注射黑胸败血芽孢杆菌致病菌和非致病菌（大肠杆菌）后的3、9、12 h,检测到脂肪体中的Bm-defA/B的转录活性上调,Bm-defB的响应活性高于Bm-defA,Bm-defB对黑胸败血芽孢杆菌致病菌的响应快于Bm-defA,提示Bm-defensins在家蚕对抗细菌侵染的免疫反应中发挥重要的作用。...

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Published in西南农业学报 Vol. 26; no. 3; pp. 1282 - 1285
Main Author 唐芬芬 杨伟克 邵榆岚 杨海 白兴荣
Format Journal Article
LanguageChinese
Published 云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101 2013
华南农业大学动物科学学院,广东广州510642%华南农业大学动物科学学院,广东广州,510642%云南省农业科学院蚕桑蜜蜂研究所,云南蒙自,661101
Subjects
B
defensinA
大肠杆菌
实时荧光定量PCR
家蚕
黑胸败血芽孢杆菌
Real-time fluorescent quantitative PCR
家蚕
黑胸败血芽孢杆菌
defensinA/B
实时荧光定量PCR
Escherichia coli
大肠杆菌
Bombyx mori
Bacillus bombyseptieus
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ISSN1001-4829

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Summary:在致病菌（Bacillus bombyseptieus）与非致病菌（Escherichia coli）诱导家蚕后的不同时间,采用实时荧光定量PCR方法,对抗菌肽基因defensinA/B在脂肪体中的表达进行了定量分析。结果表明,在家蚕被注射黑胸败血芽孢杆菌致病菌和非致病菌（大肠杆菌）后的3、9、12 h,检测到脂肪体中的Bm-defA/B的转录活性上调,Bm-defB的响应活性高于Bm-defA,Bm-defB对黑胸败血芽孢杆菌致病菌的响应快于Bm-defA,提示Bm-defensins在家蚕对抗细菌侵染的免疫反应中发挥重要的作用。
Bibliography:In this paper,the real-time fluorescent quantitative PCR was applied to analyze the expression of Bm-defensinA/B in fat body after being injected pathogens（Bacillus bombyseptieus）and non-pathogens（Escherichia coli） of Bombyx mori.The results showed that defensinA and defensinB were all up-regulated after injection with B.bombyseptieus and E.coli at 3,9 and 12 h.Moreover,the expression of defensinB was higher and faster than that of defensinA.These suggested that Bm-defensins played an important role in B.mori immune response to against infection by pathogen.
TANG Fen-fen1,2,YANG Wei-ke2,SHAO Yu-lan1,YANG Hai1,BAI Xing-rong1（1.Institute of Sericulture and Apiculture,Yunnan Academy of Agricultural Sciences,Yunnan Mengzi 661101,China;2.College of Animal Science,South China Agricultural University,Guangdong Guangzhou 510642,China）
Bombyx mori ; Bacillus bombyseptieus ; Escherichia coil; DefensinA/ B ; Real-time fluorescent quantitative PCR
51-1213/S
ISSN:1001-4829