甘蔗属热带种与滇蔗茅远缘杂交F1代GISH分析

滇蔗茅是甘蔗野生近缘属植物资源，通过光周期诱导甘蔗属热带种与野生滇蔗茅花期相遇进行远缘杂交利用，获得了远缘杂交FI代创新种质材料。本研究首先采用常规压片法对远缘杂交FI代材料云南03-316染色体数日进行鉴定，结果2n=54条；进一步的双色基因组荧光原位杂交（gen0IIIic／ns／tuhybridization，GISH）分析结果表明，杂交FI代细胞中含有15条来自父本云南95-20的染色体及39条来自母本越南牛蔗的染色体，远缘杂交过程双亲本染色体以n＋n的遗传配对方式进行传递。从F．代问期核荧光原位杂交结果可以看出，来自双亲本的染色质以分散的方式单独分布。研究结果可为F1代创新材料的后续...

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Published in西南农业学报 Vol. 26; no. 4; pp. 1327 - 1331
Main Author 林秀琴 陆鑫 毛钧 刘新龙 苏火生 蔡青
Format Journal Article
LanguageChinese
Published 云南省农业科学院生物技术与种质资源保存研究所,云南昆明650223 2013
云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室,云南开远,661699%云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室,云南开远661699
云南大学生命科学院,云南昆明650091
Subjects
F1代
基因组荧光原位杂交
滇蔗茅
甘蔗
F1hybrids
甘蔗
基因组荧光原位杂交
Saccharum officinarum
滇蔗茅
GISH
F1代
Erianthus rockii
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ISSN1001-4829

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Summary:滇蔗茅是甘蔗野生近缘属植物资源，通过光周期诱导甘蔗属热带种与野生滇蔗茅花期相遇进行远缘杂交利用，获得了远缘杂交FI代创新种质材料。本研究首先采用常规压片法对远缘杂交FI代材料云南03-316染色体数日进行鉴定，结果2n=54条；进一步的双色基因组荧光原位杂交（gen0IIIic／ns／tuhybridization，GISH）分析结果表明，杂交FI代细胞中含有15条来自父本云南95-20的染色体及39条来自母本越南牛蔗的染色体，远缘杂交过程双亲本染色体以n＋n的遗传配对方式进行传递。从F．代问期核荧光原位杂交结果可以看出，来自双亲本的染色质以分散的方式单独分布。研究结果可为F1代创新材料的后续利用提供细胞学参考价值。
Bibliography:The Erianthus rochii Ken$ is the wild relatives of sugarcane, distant hybridization was took between Saccharum o ffic/narum L. and E. rochii Keng while it＇ s flowering meet by the photoperiodic induction, and Ft generation was obtained. The chromosome number in Ft was identified with 2n = 54 by conventional pressure plate method. And the l~sult of the 8enomic in situ hybridization （GISH） showed that there were 15 E. roc/di chromosomes and 39 S. o fficinarum chromosomes in F! progeny （Yunnan03-316）. Chromosomal constitution of Fl hybrids derived from sexual cro~s between S. o.fficinarum L. and E. recJdi was n ＋ n. The GISH result on nuclear of Ft progeny showed the chrome- tins came from male and female was not intermixed and stayed at different regions. The results could provide a cytology re~erence for the fol- low-up use of the Ft generation materials.
LIN Xiu-qinI, LU XinI, MAO JunI, LIU Xin-longI, SU Huo-shengI , CAI Qing1＇2＇3 ~ （ 1. Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Y
ISSN:1001-4829