大熊猫肠道大肠杆菌的分离、鉴定及其耐药性分析

本实验分离大熊猫粪便样品中大肠杆菌,利用形态学和分子生物学方法鉴定菌株,采用药敏纸片扩散法(Kirby-Bauer)进行常用抗生素耐药性试验分析。结果表明,从抗生素大类来比较,耐药率依次为:四环素类(55.6%)〉青霉素类(26.7%)〉磺胺类(18.9%)〉喹诺酮类(10%)〉头孢类(7.8%)〉氨基糖苷类(1.1%)〉单环内酰胺类(0)、碳青霉烯类(0)。大肠杆菌对四环素的耐药率最高,达到55.6%;对头孢曲松、头孢他啶、氨曲南、亚胺配能及美罗配能等敏感,耐药率为0。其它抗菌药物耐药率依次是:阿莫西林(26.7%)〉磺胺复合物(18.9%)〉氨苄西林/舒巴坦(17.8%)〉复方新诺明(11...

Full description

Saved in:
Bibliographic Details
Published in西南农业学报 Vol. 25; no. 3; pp. 1109 - 1113
Main Author 李蓓 李旭林 郭丽娟 龙梅 郭放 何雪梅 罗燕 邹立扣
Format Journal Article
LanguageChinese
Published 四川农业大学都江堰校区微生物学实验室,森林资源保护与利用实验室,四川都江堰611830 2012
Subjects
Online AccessGet full text
ISSN1001-4829
DOI10.3969/j.issn.1001-4829.2012.03.076

Cover

More Information
Summary:本实验分离大熊猫粪便样品中大肠杆菌,利用形态学和分子生物学方法鉴定菌株,采用药敏纸片扩散法(Kirby-Bauer)进行常用抗生素耐药性试验分析。结果表明,从抗生素大类来比较,耐药率依次为:四环素类(55.6%)〉青霉素类(26.7%)〉磺胺类(18.9%)〉喹诺酮类(10%)〉头孢类(7.8%)〉氨基糖苷类(1.1%)〉单环内酰胺类(0)、碳青霉烯类(0)。大肠杆菌对四环素的耐药率最高,达到55.6%;对头孢曲松、头孢他啶、氨曲南、亚胺配能及美罗配能等敏感,耐药率为0。其它抗菌药物耐药率依次是:阿莫西林(26.7%)〉磺胺复合物(18.9%)〉氨苄西林/舒巴坦(17.8%)〉复方新诺明(11.1%)〉萘啶酸(10%)〉头孢噻吩(7.8%)〉阿莫西林/克拉维酸(3.3%)〉庆大霉素(2.2%),共产生了33种耐药谱,大部分菌株至少耐1种药物,TET为优势耐药谱(18/90),其次为TET、AML(10/90),多重耐药较普遍(40/90),个别菌株耐药谱达到6种,此研究为大熊猫肠道疾病的合理、科学治疗奠定了基础。
Bibliography:Escherichia coli was isolated fi'om Giant panda feces and identified by morphology and molecular biology method. Antimicrobial susceptibility were tested by the standardized Disk diffusion method ( Kirby-Baner). Results showed that resistance rates of tetracycline, pen- icillin, sulfonamidos, quinolanes, cephalospofins, aminoglycosides, monobactam and earbapenem were 55.6 % ,26.7 %, 18.9 %, 12.2 % ,7.8 % ,1.1% ,0,0,respectively. The E. coli strains were the most resistant to tetracycline(55.6 % ), followed by amoxieillin(26.7 % ), sulfanilamides complex ( 18.9 % ), ampicillin/sulbactam ( 13.3 % ), trimethoprim= sulfamethoxazole ( 11.1% ), nalidixic acid( 10 % ), eefalotin(7.8 % ), amoxicillin/clavulanic acid(3.3 % ) and gentamicin(2.2 % ) ,respectively. Besides,all of the isolates were sen- sitive to cezftriaxone, ceftasidime, aztreonam, imipenem and meropenem. There appeared 33 resistance spectrums. Most of the stains (40/90) were resistant to at least one drug. The TET was the most popular drug resistant sp
ISSN:1001-4829
DOI:10.3969/j.issn.1001-4829.2012.03.076