肇庆、云浮地区砂糖橘黄龙病PCR快速检测技术的建立
以砂糖橘叶脉为材料,采用微量提取法提取DNA,利用柑橘黄龙病病原的特异引物对其16S rDNA片段进行PCR扩增,将其扩增产物纯化、回收,筛选含有黄龙病病原16S rDNA片段的阳性克隆进行序列测定,初步建立了适用于肇庆、云浮地区砂糖橘黄龙病PCR检测技术,并通过该技术对肇庆、云浮地区砂糖橘黄龙病的发生情况进行了初步了解。结果表明：应用16S rDNA检测砂糖橘黄龙病在肇庆、云浮地区是可行的,肇庆、云浮地区各县砂糖橘产区均检测到感染黄龙病植株。...
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| Published in | 广东农业科学 Vol. 41; no. 6; pp. 96 - 98 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
肇庆学院生命科学学院,广东肇庆526061
2014
肇庆学院果树研究所,广东肇庆,526061%肇庆学院生命科学学院,广东肇庆,526061%肇庆学院果树研究所,广东肇庆526061 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1004-874X |
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| Abstract | 以砂糖橘叶脉为材料,采用微量提取法提取DNA,利用柑橘黄龙病病原的特异引物对其16S rDNA片段进行PCR扩增,将其扩增产物纯化、回收,筛选含有黄龙病病原16S rDNA片段的阳性克隆进行序列测定,初步建立了适用于肇庆、云浮地区砂糖橘黄龙病PCR检测技术,并通过该技术对肇庆、云浮地区砂糖橘黄龙病的发生情况进行了初步了解。结果表明：应用16S rDNA检测砂糖橘黄龙病在肇庆、云浮地区是可行的,肇庆、云浮地区各县砂糖橘产区均检测到感染黄龙病植株。 |
|---|---|
| AbstractList | 以砂糖橘叶脉为材料,采用微量提取法提取DNA,利用柑橘黄龙病病原的特异引物对其16S rDNA片段进行PCR扩增,将其扩增产物纯化、回收,筛选含有黄龙病病原16S rDNA片段的阳性克隆进行序列测定,初步建立了适用于肇庆、云浮地区砂糖橘黄龙病PCR检测技术,并通过该技术对肇庆、云浮地区砂糖橘黄龙病的发生情况进行了初步了解。结果表明：应用16S rDNA检测砂糖橘黄龙病在肇庆、云浮地区是可行的,肇庆、云浮地区各县砂糖橘产区均检测到感染黄龙病植株。 S432%S436.661; 以砂糖橘叶脉为材料,采用微量提取法提取DNA,利用柑橘黄龙病病原的特异引物对其16S rDNA片段进行PCR扩增,将其扩增产物纯化、回收,筛选含有黄龙病病原16S rDNA片段的阳性克隆进行序列测定,初步建立了适用于肇庆、云浮地区砂糖橘黄龙病PCR检测技术,并通过该技术对肇庆、云浮地区砂糖橘黄龙病的发生情况进行了初步了解.结果表明:应用16S rDNA检测砂糖橘黄龙病在肇庆、云浮地区是可行的,肇庆、云浮地区各县砂糖橘产区均检测到感染黄龙病植株. |
| Author | 郭丽英 郭雁君 麦丽瑶 岑惠锋 吴道洲 吉前华 |
| AuthorAffiliation | 肇庆学院果树研究所,广东肇庆526061 肇庆学院生命科学学院,广东肇庆526061 |
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| Author_FL | MAI Li-yao CEN Hui-feng WU Dao-zhou GUO Yan-jun GUO Li-ying JI Qian-hua |
| Author_FL_xml | – sequence: 1 fullname: GUO Li-ying – sequence: 2 fullname: GUO Yan-jun – sequence: 3 fullname: MAI Li-yao – sequence: 4 fullname: CEN Hui-feng – sequence: 5 fullname: WU Dao-zhou – sequence: 6 fullname: JI Qian-hua |
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| ClassificationCodes | S432%S436.661 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Establishment of PCR testing technique for citrus HLB in Zhaoqing and Yunfu |
| DocumentTitle_FL | Establishment of PCR testing technique for citrus HLB in Zhaoqing and Yunfu |
| EndPage | 98 |
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| Issue | 6 |
| Keywords | 肇庆 砂糖橘 PCR 黄龙病 |
| Language | Chinese |
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| Notes | 44-1267/S GUO Li-ying, GUO Yan-jun, MAI Li-yao, CEN Hui-feng, WU Dao-zhou, JI Qian-hua （1. Fruit Research hatitute, Zhaoqing University, Zhaoqing 526061, China; 2. College of Life Sciences, Zhaoqing University, Zhaoqing 526061, China） HLB;PCR;Zhaoqing;citrus The experiment was made with citrus leaves as samples and by means of DNA extraction. Specific primers from HLB agents were applied to amplify through PCR, the final product of which would be purified and recollected. 16Sr DNA segments were positively cloned and sequenced. A PCR testing technique was established for citrus HLB in Zhaoqing and Yunfu area. The PCR technique was applied to test suspected citrus HLB samples all cross Zhaoqing and Yunfu. Experiment results indicated that PCR testing technique was feasible and citrus plants with HLB infection were discovered in Zhaoqing and Yunfu. |
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| PublicationTitle | 广东农业科学 |
| PublicationTitleAlternate | Guangdong Agricultural Sciences |
| PublicationTitle_FL | Guangdong Agricultural Sciences |
| PublicationYear | 2014 |
| Publisher | 肇庆学院生命科学学院,广东肇庆526061 肇庆学院果树研究所,广东肇庆,526061%肇庆学院生命科学学院,广东肇庆,526061%肇庆学院果树研究所,广东肇庆526061 |
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| Snippet | 以砂糖橘叶脉为材料,采用微量提取法提取DNA,利用柑橘黄龙病病原的特异引物对其16S rDNA片段进行PCR扩增,将其扩增产物纯化、回收,筛选含有黄龙病病原16S rDNA片段的阳性克隆... S432%S436.661; 以砂糖橘叶脉为材料,采用微量提取法提取DNA,利用柑橘黄龙病病原的特异引物对其16S rDNA片段进行PCR扩增,将其扩增产物纯化、回收,筛选含有黄龙病病原16S... |
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| StartPage | 96 |
| SubjectTerms | PCR 砂糖橘 肇庆 黄龙病 |
| Title | 肇庆、云浮地区砂糖橘黄龙病PCR快速检测技术的建立 |
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