肇庆、云浮地区砂糖橘黄龙病PCR快速检测技术的建立

以砂糖橘叶脉为材料,采用微量提取法提取DNA,利用柑橘黄龙病病原的特异引物对其16S rDNA片段进行PCR扩增,将其扩增产物纯化、回收,筛选含有黄龙病病原16S rDNA片段的阳性克隆进行序列测定,初步建立了适用于肇庆、云浮地区砂糖橘黄龙病PCR检测技术,并通过该技术对肇庆、云浮地区砂糖橘黄龙病的发生情况进行了初步了解。结果表明：应用16S rDNA检测砂糖橘黄龙病在肇庆、云浮地区是可行的,肇庆、云浮地区各县砂糖橘产区均检测到感染黄龙病植株。...

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Published in广东农业科学 Vol. 41; no. 6; pp. 96 - 98
Main Author 郭丽英 郭雁君 麦丽瑶 岑惠锋 吴道洲 吉前华
Format Journal Article
LanguageChinese
Published 肇庆学院生命科学学院,广东肇庆526061 2014
肇庆学院果树研究所,广东肇庆,526061%肇庆学院生命科学学院,广东肇庆,526061%肇庆学院果树研究所,广东肇庆526061
Subjects
PCR
砂糖橘
肇庆
黄龙病
肇庆
砂糖橘
PCR
黄龙病
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ISSN1004-874X

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Summary:以砂糖橘叶脉为材料,采用微量提取法提取DNA,利用柑橘黄龙病病原的特异引物对其16S rDNA片段进行PCR扩增,将其扩增产物纯化、回收,筛选含有黄龙病病原16S rDNA片段的阳性克隆进行序列测定,初步建立了适用于肇庆、云浮地区砂糖橘黄龙病PCR检测技术,并通过该技术对肇庆、云浮地区砂糖橘黄龙病的发生情况进行了初步了解。结果表明：应用16S rDNA检测砂糖橘黄龙病在肇庆、云浮地区是可行的,肇庆、云浮地区各县砂糖橘产区均检测到感染黄龙病植株。
Bibliography:44-1267/S
GUO Li-ying, GUO Yan-jun, MAI Li-yao, CEN Hui-feng, WU Dao-zhou, JI Qian-hua （1. Fruit Research hatitute, Zhaoqing University, Zhaoqing 526061, China; 2. College of Life Sciences, Zhaoqing University, Zhaoqing 526061, China）
HLB;PCR;Zhaoqing;citrus
The experiment was made with citrus leaves as samples and by means of DNA extraction. Specific primers from HLB agents were applied to amplify through PCR, the final product of which would be purified and recollected. 16Sr DNA segments were positively cloned and sequenced. A PCR testing technique was established for citrus HLB in Zhaoqing and Yunfu area. The PCR technique was applied to test suspected citrus HLB samples all cross Zhaoqing and Yunfu. Experiment results indicated that PCR testing technique was feasible and citrus plants with HLB infection were discovered in Zhaoqing and Yunfu.
ISSN:1004-874X