肿柄菊水提液对蜈蚣蕨孢子萌发的影响

采用正交实验法对蜈蚣蕨孢子在人工培养基上无污染萌发的条件进行探索,研究不同部位不同浓度的肿柄菊提取液对蜈蚣蕨孢子萌发和假根生长的影响。结果表明：对孢子进行4%次氯酸钠、0.1%升汞消毒8 min,50 mg/L赤霉素1 mL处理2 min,接种于pH值为6的1/2Ms培养基上,效果最好。随着水浸液浓度的升高,肿柄菊的抑制作用不断增强,浓度为3%时,不同部位的提取液均使孢子不能萌发。对蜈蚣蕨孢子萌发抑制作用最强的是根水浸液,而对假根生长抑制作用最强的是叶提取液。结论：不同植物、同种植物不同部位的生长对同种化感作用的敏感度不同,同种植物的不同部位对植物不同生长时期的化感作用也不尽相同。...

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Bibliographic Details
Published in西南农业学报 Vol. 25; no. 4; pp. 1449 - 1454
Main Author 杨海艳 祝云 徐成东 谢美华
Format Journal Article
LanguageChinese
Published 楚雄师范学院化学与生命科学系,云南楚雄,675000 2012
Subjects
假根生长
化感作用
孢子萌发
正交实验
假根生长
孢子萌发
正交实验
化感作用
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ISSN1001-4829

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Summary:采用正交实验法对蜈蚣蕨孢子在人工培养基上无污染萌发的条件进行探索,研究不同部位不同浓度的肿柄菊提取液对蜈蚣蕨孢子萌发和假根生长的影响。结果表明：对孢子进行4%次氯酸钠、0.1%升汞消毒8 min,50 mg/L赤霉素1 mL处理2 min,接种于pH值为6的1/2Ms培养基上,效果最好。随着水浸液浓度的升高,肿柄菊的抑制作用不断增强,浓度为3%时,不同部位的提取液均使孢子不能萌发。对蜈蚣蕨孢子萌发抑制作用最强的是根水浸液,而对假根生长抑制作用最强的是叶提取液。结论：不同植物、同种植物不同部位的生长对同种化感作用的敏感度不同,同种植物的不同部位对植物不同生长时期的化感作用也不尽相同。
Bibliography:YANG Hai-yan,ZHU Yun,XU Cheng-dong,XIE Mei-hua（Department of Chemistry and Life Science,Chuxiong Normal University,Yunnan Chuxiong 675000,China）
51-1213/S
Using orthogonal test to find the pollution-free condition for spore germination of Pteris vittata on synthetic medium,the allelopathic effects of extracts from leaves,roots and stems of Tithonia diversifolia were studied.Results showed that the suitable environment for germination was 1/2 Ms medium with pH 6 and disinfected the spore 8min with 4 %NaClO and 0.1 %HgCl2,and then soaked in 1 mL 50 mg/LGA lasting to 1min before culturing them on synthetic medium.The allelopathic inhibitory effects generally increased with increasing concentrations and the spore all died when the concentration of extracts reached at 3 %.The inhibitory action to spore germination was the aqueous extracts from root while the leaf extracts had the strongest inhibitory effect on rhizoid growth.The conclusion was that different plants,different part of the same plant had different sens
ISSN:1001-4829