原料奶中微生物区系ERIC-PCR DNA指纹图谱的建立

为了研究原料奶中微生物区系的结构,该研究采用肠杆菌基因间保守重复序列ERIC-PCR技术,建立了华北和中南地区原料奶中微生物区系的ERIC-PCR DNA指纹图谱。为获得清晰且高质量的图谱,对ERIC-PCR的反应条件进行了优化,确定最佳条件为：退火温度52℃、MgCl21μL、引物0.6μL、Taq酶0.5μL。对2个地区指纹图谱进行综合分析鉴定。结果显示,地理位置距离较近即相近地域原料奶样品其微生物群落的构成相似性较高,存在一定特征性优势菌群;距离较远则相似性较低。研究结果提示,建立中国不同地区原料奶微生物区系DNA的特征图谱,将为原料奶质量与安全的监测提供参考。...

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Bibliographic Details
Published in农业工程学报 Vol. 29; no. 11; pp. 261 - 269
Main Author 陈庆森 高文茹 朱晨晨 阎亚丽 庞广昌 胡志和
Format Journal Article
LanguageChinese
Published 天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院,天津 300134%天津市天狮生命科学与技术研究院技术支持部,天津 301700 2013
Subjects
DNA指纹图谱
ERIC-PCR
优化
农产品
原料奶
微生物
microorganisms
optimization
优化
agricultural products
农产品
原料奶
raw milk
DNA fingerprint
DNA指纹图谱
微生物
ERIC-PCR
Online AccessGet full text
ISSN1002-6819
DOI10.3969/j.issn.1002-6819.2013.11.034

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Summary:为了研究原料奶中微生物区系的结构,该研究采用肠杆菌基因间保守重复序列ERIC-PCR技术,建立了华北和中南地区原料奶中微生物区系的ERIC-PCR DNA指纹图谱。为获得清晰且高质量的图谱,对ERIC-PCR的反应条件进行了优化,确定最佳条件为：退火温度52℃、MgCl21μL、引物0.6μL、Taq酶0.5μL。对2个地区指纹图谱进行综合分析鉴定。结果显示,地理位置距离较近即相近地域原料奶样品其微生物群落的构成相似性较高,存在一定特征性优势菌群;距离较远则相似性较低。研究结果提示,建立中国不同地区原料奶微生物区系DNA的特征图谱,将为原料奶质量与安全的监测提供参考。
Bibliography:11-2047/S
microorganisms; optimization; agricultural products; raw milk; ERIC-PCR; DNA fingerprint
Enterobacterial Repetitive Intergenic Consensus （ERIC） primarily exists in enterobacteria. The sequence length is about 124-127 bp, which contain about 44 bp highly conservative core sequences in its center. ERIC-PCR was widely applied to research the intestinal microbial community composition and diversity. However, we rarely saw domestic and foreign related reports about the ERIC-PCR method which was adopted to analyze the microbial community compositon in raw milk. ERIC-PCR molecular biotechnology was used to research the microbial community, and DNA fingerprints on microbes in raw milk in North China and in Central South China was established in this paper. First, the bacteria DNA extraction method in raw milk had to be built. This paper utilized the CTAB extraction method. Secondly, we needed to determine the optimal ERIC-PCR reaction condition. In order to obtain a distinct and high quality fingerprint, infl
ISSN:1002-6819
DOI:10.3969/j.issn.1002-6819.2013.11.034