金合欢组织培养技术研究

以金合欢的种子为外植体进行离体培养,初步建立了金合欢离体培养再生体系。结果表明：最适宜诱导愈伤组织和不定芽的培养基是MS＋6-BA 1.5 mg/L＋NAA 0.1 mg/L;最适宜的增殖培养基是MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L;最适宜诱导生根的培养基是1/2MS＋IBA 0.4 mg/L。...

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Bibliographic Details
Published in广东农业科学 Vol. 39; no. 2; pp. 40 - 41
Main Author 贾文庆 刘会超 郝俊富
Format Journal Article
LanguageChinese
Published 河南科技学院园林学院,河南新乡,453003 2012
Subjects
不定芽
增殖培养
无菌苗
金合欢
不定芽
无菌苗
增殖培养
金合欢
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ISSN1004-874X
DOI10.3969/j.issn.1004-874X.2012.02.015

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Summary:以金合欢的种子为外植体进行离体培养,初步建立了金合欢离体培养再生体系。结果表明：最适宜诱导愈伤组织和不定芽的培养基是MS＋6-BA 1.5 mg/L＋NAA 0.1 mg/L;最适宜的增殖培养基是MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L;最适宜诱导生根的培养基是1/2MS＋IBA 0.4 mg/L。
Bibliography:44-1267/S
The seeds were used as explant to carry out tissue culture of Acacia dealbat, the system of efficient plant regenerations was established initially. The results showed that the explants of Albizzia treated by 0.15% HgCl2 for 10 min could obtained low rate of dead and infection, straight insertion seed is best inoculation method. The optimal culture mediums for callus tissue and adventitious bud induction was MS＋6-BA 1.5 mg/L＋NAA 0.1 mg/L, the optimal enrichment medium was MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L, the optimal rooting medium was 1/2MS＋IBA 0.4 mg/L.
JIA Wen-qing, LIU Hui-chao, HAO Jun-fu （Landscape College, Henan Institute of Science and Technology, Xinxiang 453003, China）
Albizzia; aseptic seedling; adventitious bud; muhiplication culture
ISSN:1004-874X
DOI:10.3969/j.issn.1004-874X.2012.02.015