Expression of CD44, CD44v9, ABCG2, CD24, Bmi-1 and ALDH1 in stage I and II oral squamous cell carcinoma and their association with clinicopathological factors

• Published in: Oncology letters Vol. 16; no. 1; pp. 1133 - 1140
• Main Authors: Tamatani, Tetsuya, Takamaru, Natsumi, Ohe, Go, Akita, Kazuya, Nakagawa, Takayuki, Miyamoto, Youji
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.07.2018; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: aldehyde dehydrogenase 1; ATP-binding cassette sub-family G member 2; B lymphoma Mo-MLV insertion region 1 homolog; Cancer; Care and treatment; Cell cycle; Cell division; Cellular proteins; Classification; cluster of differentiation 24; cluster of differentiation 44; cluster of differentiation 44 variant 9; Dehydrogenases; Development and progression; Gene expression; Genetic aspects; Genotype & phenotype; Health aspects; Leukemia; Lymphatic system; Medical prognosis; Metastasis; Mouth cancer; Oral cancer; Proteins; Squamous cell carcinoma; Stem cells; Studies; Surgery; Tumors; cluster of differentiation 44 variant 9; cluster of differentiation 44; B lymphoma Mo-MLV insertion region 1 homolog; ATP-binding cassette sub-family G member 2; aldehyde dehydrogenase 1; cluster of differentiation 24; oral cancer
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2018.8703

Cancer stem cells (CSCs) exhibit self-replication, self-differentiation, drug resistance and immune evasion activities. In recent years CSCs have become increasingly important for the treatment of malignant tumors. CSCs express specific markers, including cluster of differentiation (CD)44, CD44 variant 9 (CD44v9), ATP-binding cassette sub-family G member 2 (ABCG2), CD24, B lymphoma Mo-MLV insertion region 1 homolog (BMI-1) and aldehyde dehydrogenase 1 (ALDH1). However, the prognostic value of their expression in patients with oral squamous cell carcinoma (OSCC) are not well known. The present study evaluated these markers in stage I and II patients with OSCC and examined the association between T classification, histological differentiation, classification of invasion mode, lymph node metastasis and disease-free survival rate. Tissue specimens were obtained from 70 patients with stage I or II OSCC following either surgery or biopsy. Immunohistochemistry was performed and positive staining was defiend as 10% positive cells. CD44 and CD44v9 expressions were strongly detected in all OSCC tissues compared with normal epithelial cells. A total of 22 (31.4%) cases expressed ABCG2 and there was a significant association between ABCG2 expression and invasion. A total of 41 cases (59.0%) expressed CD24 and there was a significant association between CD24 expression and invasion. A total of 33 cases (47.1%) expressed BMI-1 and there was a significant association between BMI-1 expression and the disease-free survival rate. A total of 18 cases (25.7%) expressed ALDH1. Although there was no association between ALDH1 expression and T classification, there were significant associations between ALDH1 expression and histological differentiation, invasion mode, metastasis and the disease-free survival rate. Multivariate analysis revealed that ALDH1 expression was the only prognostic factor for disease-free survival rate. The results of the present study suggest that the positivity of ALDH1 detected in patients with OSCC correlates with the number of cells undergoing epithelial mesenchymal transition and metastasis. These findings indicated that the expression of ALDH1 may be an effective prognostic marker indicating the survival of patients with stage I and II OSCC.