艾塞那肽对波动血糖损伤的HRMCs的保护作用及机制

目的观察胰高血糖素样肽1（GLP-1）受体激动剂类药物艾塞那肽对波动血糖培养下受损伤的人肾小球系膜细胞（HRMCs）内质网应激（ERS）的影响，并探讨其机制。方法将体外培养的HRMCs分为3组：对照组（N组，5.6mmol/L葡萄糖培养24h）、波动血糖组（F组，30mmol/L葡萄糖培养3h后5.6mmol/L葡萄糖培养2h，重复3次，5.6mmol/L葡萄糖培养过夜）、波动血糖+艾塞那肽组（F+G组，在F组基础上加用100nmol/L艾塞那肽）。MTT法测定各组细胞增殖能力；流式细胞术检测细胞凋亡率；Westernblot法测定葡萄糖调节蛋白78（GRP78）和CCAAT/增强子结合蛋白同...

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Bibliographic Details
Published in天津医药 Vol. 45; no. 7; pp. 682 - 685
Main Author 张建双;郭婷婷;谢云
Format Journal Article
LanguageChinese
Published 天津医科大学代谢病医院内分泌研究所,卫生部激素与发育重点实验室,天津市代谢性疾病重点实验室 300070 2017
Subjects
肾小球系膜细胞;细胞增殖;细胞凋亡;内质网应激;波动血糖;艾塞那肽
细胞凋亡
肾小球系膜细胞
cell proliferation
艾塞那肽
fluctuating hyperglycemia
apoptosis
波动血糖
内质网应激
mesangial cells
endoplasmic reticulum stress
细胞增殖
exenatide
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ISSN0253-9896
DOI10.11958/20170446

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Summary:目的观察胰高血糖素样肽1（GLP-1）受体激动剂类药物艾塞那肽对波动血糖培养下受损伤的人肾小球系膜细胞（HRMCs）内质网应激（ERS）的影响，并探讨其机制。方法将体外培养的HRMCs分为3组：对照组（N组，5.6mmol/L葡萄糖培养24h）、波动血糖组（F组，30mmol/L葡萄糖培养3h后5.6mmol/L葡萄糖培养2h，重复3次，5.6mmol/L葡萄糖培养过夜）、波动血糖+艾塞那肽组（F+G组，在F组基础上加用100nmol/L艾塞那肽）。MTT法测定各组细胞增殖能力；流式细胞术检测细胞凋亡率；Westernblot法测定葡萄糖调节蛋白78（GRP78）和CCAAT/增强子结合蛋白同源蛋白（CHOP）的表达水平。结果与N组相比，F组细胞增殖能力降低，细胞凋亡率升高，内质网应激相关标志分子GRP78和CHOP表达水平均升高（P＜0.05）；加用艾塞那肽干预后，与F组比较，F+G组细胞增殖水平升高，细胞凋亡率降低，同时，GRP78和CHOP表达水平均下降（P＜0.05）。结论艾塞那肽可能通过下调内质网应激水平来减轻波动血糖对HRMCs的损伤。
Bibliography:Objective To observe the effect of exenatide on endoplasmic reticulum stress(ERS)in human renal mesangial cells(HRMCs)injured by fluctuating hyperglycemia culture,and to explore the mechanism.Methods HRMCs were randomly divided into three groups:control group(group N,cells were cultured in5.6mmol/L glucose for24h),fluctuating hyperglycemia group(group F,cells were cultured in30mmol/L glucose for3h,5.6mmol/L glucose for2h,repeated three times in one day,then5.6mmol/L glucose overnight),fluctuating hyperglycemia and exenatide group(group F+G,HRMCs were cultured in fluctuating hyperglycemia and100nmol/L exenatide).MTT assay was used to measure the viability in each group.The apoptosis rates were detected by flow cytometry in three groups.The relative expression of glucose regulated protein78(GRP78)and CCAAT/enhancerbinding protein homologous protein(CHOP)were tested by Western blot assay.Results Compared with the group N,the cell proliferation level decreased,the cell apoptosis rate increased,and the expression
ISSN:0253-9896
DOI:10.11958/20170446