HPLC测定不同部位烟叶中没食子酸的含量

建立了高效液相色谱（HPLC）测定烟叶中没食子酸含量的方法。以超纯水为萃取剂,样品经超声提取后,采用C18色谱柱分离/二极管阵列检测器测定。结果表明：没食子酸在0.5-50.0 mg/L范围内的线性相关系数为0.9999,检出限为1.07μg/g,加标回收率为92.7%-101.6%,RSD〈5%（n=5）。9个烟叶样品没食子酸含量在107.0-242.8μg/g之间;不同部位烟叶没食子酸含量排序为：上部烟叶〉中部烟叶〉下部烟叶。...

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Bibliographic Details
Published in江西农业学报 Vol. 26; no. 6; pp. 81 - 83
Main Author 蔡国华 谢卫
Format Journal Article
LanguageChinese
Published 福建中烟工业有限责任公司 技术中心,福建 厦门,361012 2014
Subjects
没食子酸
烟叶
萃取
高效液相色谱法
Tobacco leaf
萃取
高效液相色谱法
Galic acid
烟叶
Extraction
没食子酸
HPLC
Online AccessGet full text
ISSN1001-8581

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Summary:建立了高效液相色谱（HPLC）测定烟叶中没食子酸含量的方法。以超纯水为萃取剂,样品经超声提取后,采用C18色谱柱分离/二极管阵列检测器测定。结果表明：没食子酸在0.5-50.0 mg/L范围内的线性相关系数为0.9999,检出限为1.07μg/g,加标回收率为92.7%-101.6%,RSD〈5%（n=5）。9个烟叶样品没食子酸含量在107.0-242.8μg/g之间;不同部位烟叶没食子酸含量排序为：上部烟叶〉中部烟叶〉下部烟叶。
Bibliography:36-1124/S
CAI Guo- hua, XIE Wei （Technology Center, China Tobacco Fujian Industrial Limited Company, Xiamen 361012, China）
A method for the determination of galic acid content in tobacco leaves was developed by using HPLC （ High Perform-ance Liquid Chromatography ） .After ultrasonic extraction by ultrapure water , the samples were separated by C 18 chromatographic column, and were detected by diode array detector .The results showed that：within the scope of 0.5-50.0 mg/L galic acid con-tent, the linear correlation coefficient was 0.9999, the low detection limit was 1.07μg/g, the standard-adding recovery was from 92.7%to 101.6%, and the RSD was less than 5% （n=5）.The contents of galic acid in 9 tobacco leaf samples ranged from 107.0 to 242.8 μg/g.The galic acid content in different parts of tobacco leaves could be arranged as follows ：upper leaf ＆gt;middle leaf ＆gt;lower leaf .
HPLC;Tobacco leaf;Galic acid;Extraction
ISSN:1001-8581