Superoxide dismutase and catalase activities in the seminal plasma of normozoospermic and asthenozoospermic beagles

• Published in: Journal of Veterinary Medical Science Vol. 69; no. 2; pp. 133 - 136
• Main Authors: Kawakami, E.(Nippon Veterinary and Life Science Univ., Musashino, Tokyo (Japan). Faculty of Veterinary Medicine), Takemura, A, Sakuma, M, Takano, M, Hirano, T, Hori, T, Tsutsui, T
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.02.2007; Japan Science and Technology Agency
• Subjects: Animals; Asthenozoospermia - blood; Asthenozoospermia - enzymology; Asthenozoospermia - pathology; Asthenozoospermia - veterinary; canine; CATALASA; CATALASE; Catalase - metabolism; CHIEN; Dog Diseases - blood; Dog Diseases - enzymology; Dog Diseases - pathology; DOGS; ESPERMATOZOO; Male; MOUVEMENT; MOVEMENT; MOVIMIENTO; PERRO; SEMEN; Semen - enzymology; seminal plasma; SOD; Sperm Count; Sperm Motility; SPERMATOZOA; Spermatozoa - abnormalities; SPERMATOZOIDE; SPERME; SUPEROXIDE DISMUTASE; Superoxide Dismutase - metabolism; SUPEROXIDO DISMUTASA; SUPEROXYDE DISMUTASE; TESTOSTERONAS; TESTOSTERONE; Testosterone - blood
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.69.133

We measured the blood plasma testosterone (T) levels and superoxide dismutase (SOD) and catalase activities in the seminal plasma of the ejaculates of 5 normal (2-5 years old) and 5 asthenozoospermic (AZ-) (3-5 years old) beagles. Sperm ejaculated by AZ-dogs was incubated for 3 hr in Eagle's MEM only (controls) or Eagle's MEM containing 100 units/ml of SOD or catalase. Sperm motility was examined during incubation. The mean (+- SE) plasma T level of the AZ-dogs (1.2 +- 0.2 ng/ml) was significantly lower than in the normal dogs (2.5 +- 0.2 ng/ml) (P0.005). The mean (+- SE) seminal plasma SOD and catalase activities (18.8 +- 1.9 and 0.5 +- 0.1 unit/g protein, respectively) were significantly lower in the AZ-dogs than in the normal dog (43.3 +- 2.5 and 2.2 +- 0.4 unit/g protein, respectively) (P0.001 and 0.01, respectively). The motility of sperm incubated in Eagle's MEM containing SOD or catalase was significantly higher than that of control sperm incubated in only Eagle's MEM after 2 or 3 hr of incubation (P0.05). The results of this study indicate that poor T secretion by the testes and low antioxidant enzyme activities are related to AZ in the dog.