QuEChERS法测定葡萄酒中乙烯菌核利方法研究
建立了QuEChERS测定葡萄酒中乙烯菌核利的方法。样品经乙腈提取，丙基乙二胺（Primary Secondary Amine，PSA）净化，冷冻离心分离，氮气吹干，最后采用气相色谱．串联质谱检测。结果表明：乙烯菌核利浓度在25～250μg·L^-1时与峰面积有良好的线性关系（R^2〉0．9900），定量限（10S／N）为1．3μg·L^-1；当添加量在25、50、250μg·L^-1时乙烯菌核利的平均回收率为87％～102％，相对标准偏差（Relative standard deviation，RSD）为3．1％-7．3％。因此，该方法定性定量结果准确，适合葡萄酒中乙烯菌核利残留的快速检测。...
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| Published in | 西南农业学报 Vol. 26; no. 2; pp. 835 - 837 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
四川省农业科学院分析测试中心,四川成都,610066
2013
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
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| Summary: | 建立了QuEChERS测定葡萄酒中乙烯菌核利的方法。样品经乙腈提取，丙基乙二胺（Primary Secondary Amine，PSA）净化，冷冻离心分离，氮气吹干，最后采用气相色谱．串联质谱检测。结果表明：乙烯菌核利浓度在25～250μg·L^-1时与峰面积有良好的线性关系（R^2〉0．9900），定量限（10S／N）为1．3μg·L^-1；当添加量在25、50、250μg·L^-1时乙烯菌核利的平均回收率为87％～102％，相对标准偏差（Relative standard deviation，RSD）为3．1％-7．3％。因此，该方法定性定量结果准确，适合葡萄酒中乙烯菌核利残留的快速检测。 |
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| Bibliography: | 51-1213/S HOU Xue,YI Sheng-guo , HAN Mei, ZHANG Yi-rong,YANG Xiao-feng （ Center of Analysis and Testing, Sichuan Academy of Agricultural Science, Sichuan Chengdu 610066, China） A QuEChERS method was developed for determination of vinclozolin in wine. The samples were extracted by acetonitrile, and cleaned up by PSA; Then the extracts was determined with a MS/MS detector based on MRM. The result showed that the linear ranges of the determination were 25 - 250μg·L^-1 （ R2 〉 0. 9900） and the limit of quantitation （10S/N） of this method were 1.3μg·L^-1. When the spiked levels were 25,50,250 μg·L^-1, the average recoveries ranged from 87 % to 102 % with RSD （n = 5 ） of 3.1% -7.3 %. This method is suitable for the detection of vinclozolin in wine. Wine; Vinclozolin; QuEChERS |
| ISSN: | 1001-4829 |