铁皮石斛组培苗生根条件优化研究
【目的】培养生根优良的铁皮石斛组培苗,提高移栽成活率。【方法】在生根培养基中添加不同激素配比（1.0～3.0mg/LNAA、0.5mg/L6-BA）进行铁皮石斛组培苗生根培养,并采取正交试验考察光照强度、pH和琼脂用量对铁皮石斛组培苗生根效果的影响。【结果】随着MS培养基中NAA浓度的升高,试管苗的鲜重和生根率呈逐渐降低的趋势,以添加NAA1.0mg/L（处理1）和NAA2.0mg/L（处理3）的试管苗长势最好。极差分析和方差分析结果表明,在3个培养条件中,对试管苗生根的影响大小表现为光照强度〉琼脂用量〉pH值,生根培养基3因素最优水平组合为光照强度2000lx,pH5.5,琼脂用量8.0g/...
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| Published in | 南方农业学报 Vol. 43; no. 11; pp. 1668 - 1671 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
三亚市南繁科学技术研究院,海南三亚,572000
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-1191 |
| DOI | 10.3969/j:issn.2095-1191.2012.11.1668 |
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| Abstract | 【目的】培养生根优良的铁皮石斛组培苗,提高移栽成活率。【方法】在生根培养基中添加不同激素配比（1.0～3.0mg/LNAA、0.5mg/L6-BA）进行铁皮石斛组培苗生根培养,并采取正交试验考察光照强度、pH和琼脂用量对铁皮石斛组培苗生根效果的影响。【结果】随着MS培养基中NAA浓度的升高,试管苗的鲜重和生根率呈逐渐降低的趋势,以添加NAA1.0mg/L（处理1）和NAA2.0mg/L（处理3）的试管苗长势最好。极差分析和方差分析结果表明,在3个培养条件中,对试管苗生根的影响大小表现为光照强度〉琼脂用量〉pH值,生根培养基3因素最优水平组合为光照强度2000lx,pH5.5,琼脂用量8.0g/L。【结论】铁皮石斛试管苗适宜的生根培养基为1/2MS＋10%香蕉泥＋NAA1.0mg/L＋2%蔗糖＋0.05%活性炭,适宜的生根条件为光照强度2000lx、琼脂用量8.0g/L、pH5.5。 |
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| AbstractList | 【目的】培养生根优良的铁皮石斛组培苗,提高移栽成活率。【方法】在生根培养基中添加不同激素配比（1.0～3.0mg/LNAA、0.5mg/L6-BA）进行铁皮石斛组培苗生根培养,并采取正交试验考察光照强度、pH和琼脂用量对铁皮石斛组培苗生根效果的影响。【结果】随着MS培养基中NAA浓度的升高,试管苗的鲜重和生根率呈逐渐降低的趋势,以添加NAA1.0mg/L（处理1）和NAA2.0mg/L（处理3）的试管苗长势最好。极差分析和方差分析结果表明,在3个培养条件中,对试管苗生根的影响大小表现为光照强度〉琼脂用量〉pH值,生根培养基3因素最优水平组合为光照强度2000lx,pH5.5,琼脂用量8.0g/L。【结论】铁皮石斛试管苗适宜的生根培养基为1/2MS＋10%香蕉泥＋NAA1.0mg/L＋2%蔗糖＋0.05%活性炭,适宜的生根条件为光照强度2000lx、琼脂用量8.0g/L、pH5.5。 S567.23; [目的]培养生根优良的铁皮石斛组培苗,提高移栽成活率.[方法]在生根培养基中添加不同激素配比(1.0～3.0 mg/L NAA、0.5 mg/L 6-BA)进行铁皮石斛组培苗生根培养,并采取正交试验考察光照强度、pH和琼脂用量对铁皮石斛组培苗生根效果的影响.[结果]随着MS培养基中NAA浓度的升高,试管苗的鲜重和生根率呈逐渐降低的趋势,以添加NAA 1.0 mg/L(处理1)和NAA 2.0 mg/L(处理3)的试管苗长势最好.极差分析和方差分析结果表明,在3个培养条件中,对试管苗生根的影响大小表现为光照强度＞琼脂用量＞pH值,生根培养基3因素最优水平组合为光照强度2000 lx,pH 5.5,琼脂用量8.0 g/L.[结论]铁皮石斛试管苗适宜的生根培养基为1/2MS+ 10％香蕉泥+NAA 1.0 mg/L+2％蔗糖+0.05％活性炭,适宜的生根条件为光照强度2000 lx、琼脂用量8.0 g/L、pH 5.5. |
| Author | 李宏杨 陈冠铭 杨志娟 赵光英 |
| AuthorAffiliation | 三亚市南繁科学技术研究院,海南三亚572000 |
| AuthorAffiliation_xml | – name: 三亚市南繁科学技术研究院,海南三亚,572000 |
| Author_FL | CHEN Guan-ming YANG Zhi-juan LI Hong-yang ZHAO Guang-ying |
| Author_FL_xml | – sequence: 1 fullname: LI Hong-yang – sequence: 2 fullname: CHEN Guan-ming – sequence: 3 fullname: YANG Zhi-juan – sequence: 4 fullname: ZHAO Guang-ying |
| Author_xml | – sequence: 1 fullname: 李宏杨 陈冠铭 杨志娟 赵光英 |
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| ClassificationCodes | S567.23 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Rooting condition optimization of Dendrobium plantlets |
| DocumentTitle_FL | Rooting condition optimization of Dendrobium offcinale plantlets |
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| Keywords | 光照强度 组培苗 铁皮石斛 生根条件 优化 |
| Language | Chinese |
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| Notes | 45-1381/S LI Hong-yang, CHEN Guan-ming, YANG Zhi-juan, ZHAO Guang-ying （Sanya Science and Technology Academy of Hainan National Breeding and Multiplication, Sanya, Hainan 572000, China） Objective ]This research cultured excellent Dendrobium officinale plantlets in order to improve the survival rates of transplanting plantlets. [ Method ] In the rooting culture medium of Dendrobium officinale with different hormone combinations （1.0-3.0 mg/L NAA, 0.5 mg/L 6-BA）, the orthogonal experiments of light intensity, pH, and agar amounts were adopted to test the Dendrobium o[Eeinale rooting effects. [Result]As the NAA concentration in MS medium increased, the fresh weight of test-tube plantlet and its rooting rate gradually decreased, and adding NAA 1.0 mg/L （treatment 1 ） and NAA 2.0 mg/L （treatment 3） were optimal for test-tube plantlet growth. From the range and variance analysis results, under the 3 culturing conditions, the effects on Dendrobium offlcinale were ranked in the order of illumination〉agar amount〉pH valu |
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| PublicationTitle | 南方农业学报 |
| PublicationTitleAlternate | Journal of Southern Agriculture |
| PublicationTitle_FL | Guangxi Agricultural Sciences |
| PublicationYear | 2012 |
| Publisher | 三亚市南繁科学技术研究院,海南三亚,572000 |
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| Snippet | 【目的】培养生根优良的铁皮石斛组培苗,提高移栽成活率。【方法】在生根培养基中添加不同激素配比（1.0～3.0mg/LNAA、0.5mg/L6-BA）进行铁皮石斛组培苗生根培养,并采取正交试验... S567.23; [目的]培养生根优良的铁皮石斛组培苗,提高移栽成活率.[方法]在生根培养基中添加不同激素配比(1.0～3.0 mg/L NAA、0.5 mg/L 6-BA)进行铁皮石斛组培苗生根培养,并采... |
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| SubjectTerms | 优化 光照强度 生根条件 组培苗 铁皮石斛 |
| Title | 铁皮石斛组培苗生根条件优化研究 |
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