铁皮石斛组培苗生根条件优化研究

【目的】培养生根优良的铁皮石斛组培苗,提高移栽成活率。【方法】在生根培养基中添加不同激素配比（1.0～3.0mg/LNAA、0.5mg/L6-BA）进行铁皮石斛组培苗生根培养,并采取正交试验考察光照强度、pH和琼脂用量对铁皮石斛组培苗生根效果的影响。【结果】随着MS培养基中NAA浓度的升高,试管苗的鲜重和生根率呈逐渐降低的趋势,以添加NAA1.0mg/L（处理1）和NAA2.0mg/L（处理3）的试管苗长势最好。极差分析和方差分析结果表明,在3个培养条件中,对试管苗生根的影响大小表现为光照强度〉琼脂用量〉pH值,生根培养基3因素最优水平组合为光照强度2000lx,pH5.5,琼脂用量8.0g/...

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Bibliographic Details
Published in南方农业学报 Vol. 43; no. 11; pp. 1668 - 1671
Main Author 李宏杨 陈冠铭 杨志娟 赵光英
Format Journal Article
LanguageChinese
Published 三亚市南繁科学技术研究院,海南三亚,572000 2012
Subjects
优化
光照强度
生根条件
组培苗
铁皮石斛
光照强度
组培苗
铁皮石斛
生根条件
优化
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2012.11.1668

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Summary:【目的】培养生根优良的铁皮石斛组培苗,提高移栽成活率。【方法】在生根培养基中添加不同激素配比（1.0～3.0mg/LNAA、0.5mg/L6-BA）进行铁皮石斛组培苗生根培养,并采取正交试验考察光照强度、pH和琼脂用量对铁皮石斛组培苗生根效果的影响。【结果】随着MS培养基中NAA浓度的升高,试管苗的鲜重和生根率呈逐渐降低的趋势,以添加NAA1.0mg/L（处理1）和NAA2.0mg/L（处理3）的试管苗长势最好。极差分析和方差分析结果表明,在3个培养条件中,对试管苗生根的影响大小表现为光照强度〉琼脂用量〉pH值,生根培养基3因素最优水平组合为光照强度2000lx,pH5.5,琼脂用量8.0g/L。【结论】铁皮石斛试管苗适宜的生根培养基为1/2MS＋10%香蕉泥＋NAA1.0mg/L＋2%蔗糖＋0.05%活性炭,适宜的生根条件为光照强度2000lx、琼脂用量8.0g/L、pH5.5。
Bibliography:45-1381/S
LI Hong-yang, CHEN Guan-ming, YANG Zhi-juan, ZHAO Guang-ying （Sanya Science and Technology Academy of Hainan National Breeding and Multiplication, Sanya, Hainan 572000, China）
Objective ]This research cultured excellent Dendrobium officinale plantlets in order to improve the survival rates of transplanting plantlets. [ Method ] In the rooting culture medium of Dendrobium officinale with different hormone combinations （1.0-3.0 mg/L NAA, 0.5 mg/L 6-BA）, the orthogonal experiments of light intensity, pH, and agar amounts were adopted to test the Dendrobium o[Eeinale rooting effects. [Result]As the NAA concentration in MS medium increased, the fresh weight of test-tube plantlet and its rooting rate gradually decreased, and adding NAA 1.0 mg/L （treatment 1 ） and NAA 2.0 mg/L （treatment 3） were optimal for test-tube plantlet growth. From the range and variance analysis results, under the 3 culturing conditions, the effects on Dendrobium offlcinale were ranked in the order of illumination〉agar amount〉pH valu
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2012.11.1668