连香树快速繁殖及生根过程中内源激素含量的测定

以种子为外植体对连香树的快速繁殖进行了研究,同时测定生根过程中内源激素含量的变化。结果表明：连香树种子的最佳消毒方法为0.1%升汞消毒6 min;最佳增殖培养基为MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L＋GA31.0 mg/L＋蔗糖30 g/L＋琼脂7.0g/L＋pH 6.0;最佳生根培养基为1/2MS＋IBA 2.0 mg/L＋NAA 0.5 mg/L＋6-BA 0.5 mg/L＋蔗糖30 g/L＋琼脂7.0 g/L＋pH 6.0;高浓度IAA,低浓度GA3、CTK、ABA有利于连香树组培苗不定根的形成。...

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Bibliographic Details
Published in广东农业科学 Vol. 39; no. 8; pp. 1 - 3
Main Author 陈荣珠 沈应柏 罗晓芳 张俊琦
Format Journal Article
LanguageChinese
Published 北京林业大学生物科学与技术学院,北京,100083 2012
Subjects
快速繁殖
植物内源激素
种子
连香树
连香树
种子
植物内源激素
快速繁殖
Online AccessGet full text
ISSN1004-874X
DOI10.3969/j.issn.1004-874X.2012.08.001

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Summary:以种子为外植体对连香树的快速繁殖进行了研究,同时测定生根过程中内源激素含量的变化。结果表明：连香树种子的最佳消毒方法为0.1%升汞消毒6 min;最佳增殖培养基为MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L＋GA31.0 mg/L＋蔗糖30 g/L＋琼脂7.0g/L＋pH 6.0;最佳生根培养基为1/2MS＋IBA 2.0 mg/L＋NAA 0.5 mg/L＋6-BA 0.5 mg/L＋蔗糖30 g/L＋琼脂7.0 g/L＋pH 6.0;高浓度IAA,低浓度GA3、CTK、ABA有利于连香树组培苗不定根的形成。
Bibliography:Cercidiphyllum japonicum; seed; rapid propagation; endogenous hormones
In this paper,the rapid propagation of Cercidiphyllum japonicum and the change of endogenous hormones in rooting culture were researched.The result showed that MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L＋GA3 1.0 mg/L＋cane sugar 30 g/L＋agar 7.0 g/L＋pH 6.0 was optimum for multiplication;1/2MS＋IBA 2.0 mg/L＋NAA 0.5mg/L＋6-BA 0.5 mg/L＋cane sugar 30 g/L＋agar 7.0 g/L＋ pH 6.0 was optimum medium for root culture;the high level of IAA and low level of GA3,CTK,ABA can be in favor of rooting.
44-1267/S
CHEN Rong-zhu,SHEN Ying-bai,LUO Xiao-fang,ZHANG Jun-qi（College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China）
ISSN:1004-874X
DOI:10.3969/j.issn.1004-874X.2012.08.001