香蕉枯萎病4号小种T-DNA插入突变体Focr4-1453表型特征及致病性分析
尖孢镰刀菌古巴转化型是引起香蕉枯萎病的主要病原菌,呈世界多态性分布,其致病机理尚不清楚.Focr4-1453为T-DNA插入突变体库中筛选出的一株与致病性相关的突变体.在克隆出其失活基因之后,将野生型菌株中的相同基因进行敲除和互补,得到敲除突变体ΔFoc4-1453和互补突变体ΔFoc4-1453-cp-1.对获得的3株突变体进行生物学表型研究发现,这些菌株的菌落生长速度、产孢能力以及孢子萌发率较野生型菌株明显降低,且无法透过玻璃纸进行生长.致病性测定试验表明,该基因失活会导致病原菌侵染能力显著减弱....
Saved in:
| Published in | 广东农业科学 Vol. 41; no. 12; pp. 85 - 88 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国热带农业科学院海口实验站/香蕉研究所,海南海口571101%中国热带农业科学院海口实验站/香蕉研究所,海南海口,571101%中国热带农业科学院热带生物技术研究所,海南海口,571101
2014
海南大学环境与植物保护学院,海南海口,570228%中国热带农业科学院热带生物技术研究所,海南海口571101 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1004-874X |
Cover
| Summary: | 尖孢镰刀菌古巴转化型是引起香蕉枯萎病的主要病原菌,呈世界多态性分布,其致病机理尚不清楚.Focr4-1453为T-DNA插入突变体库中筛选出的一株与致病性相关的突变体.在克隆出其失活基因之后,将野生型菌株中的相同基因进行敲除和互补,得到敲除突变体ΔFoc4-1453和互补突变体ΔFoc4-1453-cp-1.对获得的3株突变体进行生物学表型研究发现,这些菌株的菌落生长速度、产孢能力以及孢子萌发率较野生型菌株明显降低,且无法透过玻璃纸进行生长.致病性测定试验表明,该基因失活会导致病原菌侵染能力显著减弱. |
|---|---|
| Bibliography: | YUAN Gui-xiang, ZENG Tao, GUO Su-xia, CHEN Han-qing, ZENG Hui-cai （1.College of Environment and Plant Protection, Hainan University, Haikou 570228, China; 2.1nstitute of Tropical Bioseience and Bioteehnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 3.Experimental Station ~f Haikou, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China） 44-1267/S Banana fusarium-wilt disease was caused by Fusarium oxysporum f.sp. cubense and it＇t pathogenesis is unclear. Focr4-1453 is a pathogenicity-related mutant strain and it is found from T-DNA library. We got the mutant gene cDNA full length, then knockouted and complemented this mutant gene from wild-type strain and named them ΔFoc4- 1453 and ΔFoc4-1453-cp - 1. The result showed that the biological phenotype of Foc r4-1453＇ ΔFoc 4-1453 and ΔFoc 4 - 1453-cp-1 changed. Their colony growth rates, spore production capacity and spore germination were lower than those of wild-tvne strain. In addition, their virulence siznificant |
| ISSN: | 1004-874X |