新型植物活性剂对桉树愈伤诱导及芽分化的影响

以广州1号桉无菌苗的茎段作为外植体,诱导愈伤组织形成和不定芽的分化。通过对比发现,新型植物活性剂(FLC)在诱导愈伤组织和不定芽分化中均分别比TDZ和6-BA更有效,同时还确立了广州1号桉树无性系再生的最适宜培养条件:愈伤组织诱导培养基为1/2MB+FLC0.5mg/L+NAAO.1mg/L+Vc100mg/L+蔗糖30∥L+琼脂7g/L;不定芽诱导培养基为1/2MB+FLC0.6mg/L+IBA0.04mg/L+Vc100rag/L+蔗糖30g/L+琼脂7g/L。...

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Published in广东农业科学 Vol. 38; no. 11; pp. 53 - 55
Main Author 谭刻勤 欧阳乐军 李佩华 谭丽丽 鲍雁林 曾少玲 曾富华 陈信波
Format Journal Article
LanguageChinese
Published 湖南农业大学生命科学与技术学院,湖南,长沙,410128 2011
湛江师范学院生命科学与技术学院,广东,湛江,524048%湛江师范学院生命科学与技术学院,广东,湛江,524048%湛江市林业科学研究所,广东,湛江,524037%湖南农业大学生命科学与技术学院,湖南,长沙,410128
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ISSN1004-874X
DOI10.3969/j.issn.1004-874X.2011.11.020

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Summary:以广州1号桉无菌苗的茎段作为外植体,诱导愈伤组织形成和不定芽的分化。通过对比发现,新型植物活性剂(FLC)在诱导愈伤组织和不定芽分化中均分别比TDZ和6-BA更有效,同时还确立了广州1号桉树无性系再生的最适宜培养条件:愈伤组织诱导培养基为1/2MB+FLC0.5mg/L+NAAO.1mg/L+Vc100mg/L+蔗糖30∥L+琼脂7g/L;不定芽诱导培养基为1/2MB+FLC0.6mg/L+IBA0.04mg/L+Vc100rag/L+蔗糖30g/L+琼脂7g/L。
Bibliography:44-1267/S
Stem from Eucalyptus Guangzhou No.1 was taken as explants to induce callus and buds differentiation. By comparison, the new plant active agent (FLC) in the induction of callus, adventitious buds were more effective than both TDZ, 6-BA respectively. The suitable mediums of callus induction, adventitious bud induction were 1/2MB+FLC 0.5 mg/L+NAA 0.1mg/L+Vc 100 mg/L+Sucrose 30 g/L+Agar 7 g/L, 1/2MB+FLC 0.6 mg/L+IBA 0.04 mg/L+Vc 100 mg/L+Suerose 30 g/L+Agar 7 g/L.
TAN Ke-qin, OUYANG Le-jun, LI Pei-hua, TAN Li-li, BAO Yan-lin, ZENG Shao-ling, ZENG Fu-hua, CHEN Xin-bo (1.School of Life Science and Technology ,Hunan Agricultural University , Changsha 410128, China 2.School of Life Science and Technology,Zhanjiang Normal University,Zhanjiang 524048, China; 3.Zhanjiang Forestry Institute ,Zhanjiang 524037, China)
Eucalyptus Guangzhou No. 1 ; new plant active agent ; Tissue culture
ISSN:1004-874X
DOI:10.3969/j.issn.1004-874X.2011.11.020