凉粉草遗传多样性的SCoT和ISSR分析
采用SCoT和ISSR两种分子标记技术对7份凉粉草种质资源进行遗传多样性分析。结果显示：筛选出的8条SCoT引物共扩增出90个位点,多态性比率为83.33%,遗传相似系数变化范围为0.47～0.83;筛选的10条ISSR引物共扩增出100个位点,多态性比率为70.0%,相似系数变化范围为0.61～0.80。聚类分析结果显示,7份凉粉草种质资源被分为三大类,两种分子标记的分类结果基本一致,表明SCoT和ISSR两种分子标记均适用于凉粉草种质资源的遗传多样性研究。...
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| Published in | 西南农业学报 Vol. 25; no. 5; pp. 1834 - 1840 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
广西大学农学院,广西南宁,530004%广西壮族自治区优质农产品开发服务中心,广西南宁,530022%广西壮族自治区食品药品检验所,广西南宁,530021%广西壮族自治区南宁食品药品检验所,广西南宁,530001
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
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| Abstract | 采用SCoT和ISSR两种分子标记技术对7份凉粉草种质资源进行遗传多样性分析。结果显示：筛选出的8条SCoT引物共扩增出90个位点,多态性比率为83.33%,遗传相似系数变化范围为0.47～0.83;筛选的10条ISSR引物共扩增出100个位点,多态性比率为70.0%,相似系数变化范围为0.61～0.80。聚类分析结果显示,7份凉粉草种质资源被分为三大类,两种分子标记的分类结果基本一致,表明SCoT和ISSR两种分子标记均适用于凉粉草种质资源的遗传多样性研究。 |
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| AbstractList | 采用SCoT和ISSR两种分子标记技术对7份凉粉草种质资源进行遗传多样性分析。结果显示：筛选出的8条SCoT引物共扩增出90个位点,多态性比率为83.33%,遗传相似系数变化范围为0.47～0.83;筛选的10条ISSR引物共扩增出100个位点,多态性比率为70.0%,相似系数变化范围为0.61～0.80。聚类分析结果显示,7份凉粉草种质资源被分为三大类,两种分子标记的分类结果基本一致,表明SCoT和ISSR两种分子标记均适用于凉粉草种质资源的遗传多样性研究。 S647; 采用SCoT和ISSR两种分子标记技术对7份凉粉草种质资源进行遗传多样性分析.结果显示:筛选出的8条SCoT引物共扩增出90个位点,多态性比率为83.33％,遗传相似系数变化范围为0.47 ～0.83；筛选的10条ISSR引物共扩增出100个位点,多态性比率为70.0％,相似系数变化范围为0.61 ～0.80.聚类分析结果显示,7份凉粉草种质资源被分为三大类,两种分子标记的分类结果基本一致,表明SCoT和ISSR两种分子标记均适用于凉粉草种质资源的遗传多样性研究. |
| Author | 李晓晖 黎颖菁 黄荣韶 蒋明廉 王海华 |
| AuthorAffiliation | 广西大学农学院,广西南宁530004 广西壮族自治区优质农产品开发服务中心,广西南宁530022 广西壮族自治区食品药品检验所,广西南宁530021 广西壮族自治区南宁食品药品检验所,广西南宁53000 |
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| Author_FL | LI Xiao-hui LI Ying-jing HUANG Rong-shao JIANG Ming-lian WANG Hai-hua |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | SCoT and ISSR Analysis of Genetic Diversity of Mesona chinensis |
| DocumentTitle_FL | SCoT and ISSR Analysis of Genetic Diversity of Mesona chinensis |
| EndPage | 1840 |
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| Keywords | 遗传多样性分析 SCoT 凉粉草 ISSR |
| Language | Chinese |
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| Notes | 51-1213/S LI Xiao-hui , LI Ying-jing, HUANG Bong-shao , JIANG Ming-lian , WANG Hai-hua4 （ 1A@＇icultural College, GuangxJ University, Guangxl Nanning 530004, China; 2. Guangxi High-quality Agricultural Development Services Center, Guangxi Nanning 530022, China; 3. Guangxi Institute for Food and Drug Control, Guangxi Nanning 530021, China; 4. Nanning Institute for Food and Drug Control, Guangxi Nanning 530001, China ） Mesona chinensis; SCOT; ISSR; genetic diversity The genetic diversity of 7 Mesona varieties was analyzed by SCoT and ISSR. The results showed that a total of 90 loci were genera- ted by 8 SCoT primer combinations, of which 83.33 % was polymorphic and the genetic similarity ranged from 0.47 to 0.83. As for ISSR, 100 bands were generated by 10 ISSR primers, of which 70 % was polymoiphic and the genetic similarity ranged from 0.61 to 0.80. Cluste- ring analysis indicated that 7 individuals of mesona were classified into three groups. Classification results of SCoT and ISSR were basically identical, and |
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| PublicationTitle | 西南农业学报 |
| PublicationTitleAlternate | Southwest China Journal of Agricultural Sciences |
| PublicationTitle_FL | Southwest China Journal of Agricultural Sciences |
| PublicationYear | 2012 |
| Publisher | 广西大学农学院,广西南宁,530004%广西壮族自治区优质农产品开发服务中心,广西南宁,530022%广西壮族自治区食品药品检验所,广西南宁,530021%广西壮族自治区南宁食品药品检验所,广西南宁,530001 |
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| Snippet | 采用SCoT和ISSR两种分子标记技术对7份凉粉草种质资源进行遗传多样性分析。结果显示：筛选出的8条SCoT引物共扩增出90个位点,多态性比率为83.33%,遗传相似系数变化范围... S647; 采用SCoT和ISSR两种分子标记技术对7份凉粉草种质资源进行遗传多样性分析.结果显示:筛选出的8条SCoT引物共扩增出90个位点,多态性比率为83.33％,遗传相似系数变化范围... |
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| SubjectTerms | ISSR SCoT 凉粉草 遗传多样性分析 |
| Title | 凉粉草遗传多样性的SCoT和ISSR分析 |
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