凉粉草遗传多样性的SCoT和ISSR分析

采用SCoT和ISSR两种分子标记技术对7份凉粉草种质资源进行遗传多样性分析。结果显示：筛选出的8条SCoT引物共扩增出90个位点,多态性比率为83.33%,遗传相似系数变化范围为0.47～0.83;筛选的10条ISSR引物共扩增出100个位点,多态性比率为70.0%,相似系数变化范围为0.61～0.80。聚类分析结果显示,7份凉粉草种质资源被分为三大类,两种分子标记的分类结果基本一致,表明SCoT和ISSR两种分子标记均适用于凉粉草种质资源的遗传多样性研究。...

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Bibliographic Details
Published in西南农业学报 Vol. 25; no. 5; pp. 1834 - 1840
Main Author 李晓晖 黎颖菁 黄荣韶 蒋明廉 王海华
Format Journal Article
LanguageChinese
Published 广西大学农学院,广西南宁,530004%广西壮族自治区优质农产品开发服务中心,广西南宁,530022%广西壮族自治区食品药品检验所,广西南宁,530021%广西壮族自治区南宁食品药品检验所,广西南宁,530001 2012
Subjects
ISSR
SCoT
凉粉草
遗传多样性分析
遗传多样性分析
SCoT
凉粉草
ISSR
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ISSN1001-4829

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Summary:采用SCoT和ISSR两种分子标记技术对7份凉粉草种质资源进行遗传多样性分析。结果显示：筛选出的8条SCoT引物共扩增出90个位点,多态性比率为83.33%,遗传相似系数变化范围为0.47～0.83;筛选的10条ISSR引物共扩增出100个位点,多态性比率为70.0%,相似系数变化范围为0.61～0.80。聚类分析结果显示,7份凉粉草种质资源被分为三大类,两种分子标记的分类结果基本一致,表明SCoT和ISSR两种分子标记均适用于凉粉草种质资源的遗传多样性研究。
Bibliography:51-1213/S
LI Xiao-hui , LI Ying-jing, HUANG Bong-shao , JIANG Ming-lian , WANG Hai-hua4 （ 1A@＇icultural College, GuangxJ University, Guangxl Nanning 530004, China; 2. Guangxi High-quality Agricultural Development Services Center, Guangxi Nanning 530022, China; 3. Guangxi Institute for Food and Drug Control, Guangxi Nanning 530021, China; 4. Nanning Institute for Food and Drug Control, Guangxi Nanning 530001, China ）
Mesona chinensis; SCOT; ISSR; genetic diversity
The genetic diversity of 7 Mesona varieties was analyzed by SCoT and ISSR. The results showed that a total of 90 loci were genera- ted by 8 SCoT primer combinations, of which 83.33 % was polymorphic and the genetic similarity ranged from 0.47 to 0.83. As for ISSR, 100 bands were generated by 10 ISSR primers, of which 70 % was polymoiphic and the genetic similarity ranged from 0.61 to 0.80. Cluste- ring analysis indicated that 7 individuals of mesona were classified into three groups. Classification results of SCoT and ISSR were basically identical, and
ISSN:1001-4829