Isoflavone aglycon produced by culture of soybean extracts with basidiomycetes and its anti-angiogenic activity

• Published in: Bioscience, biotechnology, and biochemistry Vol. 66; no. 12; pp. 2626 - 2631
• Main Authors: Miura, T. (Amino Up Chemical Co. Ltd., Sapporo (Japan)), Yuan, L, Sun, B, Fujii, H, Yoshida, M, Wakame, K, Kosuna, K
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.12.2002; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Adult; aglycons; Air Sacs - blood supply; Air Sacs - drug effects; angiogenesis; Angiogenesis Inhibitors - chemistry; Angiogenesis Inhibitors - pharmacology; Angiogenesis Inhibitors - therapeutic use; Animals; Antineoplastic agents; basidiomycetes; beta-Glucosidase - metabolism; Biological and medical sciences; biotechnology; blood serum; Chick Embryo; chicks; Colonic Neoplasms - blood supply; Colonic Neoplasms - drug therapy; colorectal neoplasms; CULTURE TECHNIQUES; EXTRACTS; Female; FLAVONOIDS; GANODERMA LUCIDUM; General aspects; GENISTEIN; Genistein - blood; Genistein - chemistry; Genistein - pharmacology; Genistein - therapeutic use; GLUCOSIDASES; Glycine max - chemistry; glycosides; Humans; Hydrogen-Ion Concentration; isoflavone aglycon; isoflavone glycosides; Isoflavones - analysis; Isoflavones - metabolism; Isoflavones - pharmacology; Male; Medical sciences; Mice; Mice, Inbred BALB C; MORPHOGENESIS; MYCELIUM; Neovascularization, Pathologic - drug therapy; Pharmacology. Drug treatments; Plant Extracts - chemistry; Plant Extracts - metabolism; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; Polysaccharides - therapeutic use; Reishi - chemistry; Reishi - enzymology; SOYBEANS; Tumor Cells, Cultured; VASCULAR TISSUES; β-glucosidase; Cell culture; isoflavone aglycon; Basidiomycetes; Extract; Soybean; Isoflavone derivatives; Fungi; genistein; Glycoside; Aves; Antiangiogenic agent; Ganoderma; Thallophyta; Reaction product; angiogenesis; Rodentia; β-glucosidase; In vitro; Biological activity; Hydrolysis; Vertebrata; Mammalia; Mouse; Animal; Plant origin; Chicken
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.66.2626

Soybean extracts (SBE) containing isoflavone glycosides were cultured with Ganoderma lucidum mycelia producing β-glucosidase. The anti-angiogenic effects of the cultivated product, containing rich in genistein, named GCP (genistein combined polysaccharide), were assessed with chick chorioallantoic membranes (CAM) and a mouse dorsal air-sac model. β-Glucosidase produced by the mycelia converted the isoflavone glycosides into aglycons. A test of volunteers showed that serum concentrations of genistein in the subjects treated with GCP (n=4) at 3 h after administration were significantly higher than those in the subjects treated with SBE (n=4). GCP inhibited angiogenesis in CAM, and the activity of GCP was greater than that of SBE. GCP inhibited the formation of new vessels induced by colon carcinoma cells in vivo.