Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue

• Published in: Surgery for obesity and related diseases Vol. 12; no. 2; pp. 257 - 263
• Main Authors: González-Plaza, Juan José, Gutiérrez-Repiso, Carolina, García-Serrano, Sara, Rodriguez-Pacheco, Francisca, Garrido-Sánchez, Lourdes, Santiago-Fernández, Concepción, García-Arnés, Juan, Moreno-Ruiz, Francisco J., Rodríguez-Cañete, Alberto, García-Fuentes, Eduardo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2016
• Subjects: Bariatric surgery; Biopsy; Expression analysis; Female; Functional annotation; Gastric Bypass; Gastroenterology and Hepatology; Gene Expression Regulation; Humans; Lipid Metabolism - genetics; Lipids - biosynthesis; Lipids - genetics; Microarray; Morbid obesity; Obesity, Morbid - surgery; Real-Time Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Subcutaneous adipose tissue; Subcutaneous Fat - metabolism; Subcutaneous Fat - pathology; Surgery; Transcription, Genetic; Transcriptomics; Weight Loss - genetics; Expression analysis; Transcriptomics; Bariatric surgery; Gastric bypass; Functional annotation; Subcutaneous adipose tissue; Morbid obesity; Microarray
• ISSN: 1550-7289; 1878-7533; 1878-7533
• DOI: 10.1016/j.soard.2015.07.007

The changes in the transcriptomic profiling of subcutaneous adipose tissue (SAT) when weight loss stabilizes after a Roux-en-Y gastric bypass (RYGB) are still largely unknown. To investigate the changes produced in SAT gene expression of morbidly obese women when their weight loss stabilizes 2 years after RYGB. University hospital. SAT biopsies of the periumbilical area were taken before and 2 years after RYGB. Gene expression levels were assessed by microarray analysis and significant differences in gene expression were validated by real-time quantitative polymerase chain reaction. The findings were also confirmed in an independent population of morbidly obese women. Microarray analysis revealed that the overexpressed differentially expressed genes have a prominent role in the pathways involved in biosynthetic processes, especially lipid or carboxylic ones (stearoyl-Coenzyme A desaturase-1, fatty acid desaturase-1, fatty acid elongase-6, ATP citrate lyase, fatty acid synthase, lipin-1, monoacylglycerol O-acyltransferase, patatin-like phospholipase domain containing-3, phosphate cytidylyltransferase-2, cholesteryl ester transfer protein, transmembrane 7 superfamily member 2, pyruvate carboxylase, and glycogen synthase 2). Most of the underexpressed differentially expressed genes are related with immune system and inflammation processes (immune responses, response to stress, cell death, regulation of biological quality, immune effector process, the response to endogenous stimulus, and the response to other types of stimulus). An improvement of the SAT inflammatory and immune profile and an induction of genes involved in the regulation of lipid metabolism are shown when weight loss stabilizes 2 years after RYGB. Most of the genes shown are clearly linked to obesity and other metabolic disorders.