人参皂苷Rg1对局灶性脑缺血大鼠PARP-1和TNFR1表达的影响

目的探讨人参皂苷Rg1对局灶性脑缺血大脑皮质多聚二磷酸腺苷核糖聚合酶（PARP）－1和肿瘤坏死因子受体（TNFR）1表达的影响。方法90只健康SD大鼠随机均分为假手术组，单纯缺血组，人参皂苷Rg1低、中、高组，阳性对照组。于术前5d至取材当日，假手术组与单纯缺血组分别腹腔注射生理盐水45mg／kg，人参皂苷Rg1分别给10、20、40mg／kg，阳性对照组尼莫地平1mg／kg。采用大脑中动脉栓塞法制备局灶性脑缺血模型，神经功能缺损评分和TTC染色验证模型是否成功；采用免疫组织化学法和蛋白免疫印迹法检测大脑皮质缺血后PARP－1、TN－FR1的表达。结果单纯缺血组较假手术组可见明显神经功能缺陷症...

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Bibliographic Details
Published in天津医药 Vol. 43; no. 3; pp. 245 - 248
Main Author 于洋 刘学政 包翠芬 李晓明 刘霞
Format Journal Article
LanguageChinese
Published 辽宁医学院解剖学教研室 邮编121000%省高校分子细胞生物与新药开发重点实验室%组织胚胎学教研室 2015
Subjects
PARP-1
TNFR1
人参皂甙
人参皂苷Rg1
受体
型
局灶性脑缺血
聚ADP核糖聚合酶类
肿瘤坏死因子
脑缺血
受体,肿瘤坏死因子,Ⅰ型
脑缺血
brain ischemia
局灶性脑缺血
聚ADP核糖聚合酶类
人参皂甙
PARP-1
TNFR1
GINSENOSIDE
ginsenoside Rg1
poly(ADP-ribose) polymerases
focal cerebral ischemia
人参皂苷Rg1
receptors,tumor necrosis factor,type I
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ISSN0253-9896
DOI10.3969/j.issn.0253-9896.2015.03.006

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Summary:目的探讨人参皂苷Rg1对局灶性脑缺血大脑皮质多聚二磷酸腺苷核糖聚合酶（PARP）－1和肿瘤坏死因子受体（TNFR）1表达的影响。方法90只健康SD大鼠随机均分为假手术组，单纯缺血组，人参皂苷Rg1低、中、高组，阳性对照组。于术前5d至取材当日，假手术组与单纯缺血组分别腹腔注射生理盐水45mg／kg，人参皂苷Rg1分别给10、20、40mg／kg，阳性对照组尼莫地平1mg／kg。采用大脑中动脉栓塞法制备局灶性脑缺血模型，神经功能缺损评分和TTC染色验证模型是否成功；采用免疫组织化学法和蛋白免疫印迹法检测大脑皮质缺血后PARP－1、TN－FR1的表达。结果单纯缺血组较假手术组可见明显神经功能缺陷症状及大面积苍白色梗死区域；人参皂苷Rg1各组和阳性对照组神经功能评分和脑梗死体积百分比高于假手术组，低于单纯缺血组（P〈0．05）；人参皂苷Rg1各组与阳性对照组差异无统计学意义。人参皂苷Rg1低组每高倍视野PARP－1、TNFR1阳性细胞数高于假手术组和阳性对照组；中、高组高于假手术组，低于单纯缺血组（P〈0．05）。单纯缺血组皮质PARP－1、TNFR1较假手术组阳性表达条带显著增强；人参皂苷Rg1低组PARP－1、TNFR1阳性表达高于假手术组；中组高于假手术组，低于单纯缺血组；高组低于单纯缺血组（P〈0．05）。结论人参皂苷Rg1对大鼠局灶性脑缺血具有保护作用，其机制可能与下调大脑组织PARP－1、TNFR1的表达，对抗脑细胞坏死有关。
Bibliography:Objective To explore effects of ginsenosides Rgl on the expression of poly（ADP- ribose） polymerase- 1 （PARP- 1） and tumor necrosis factor receptor （TNFR） 1 in cortex cells after focal cerebral ischemia in rats. Methods Ninety healthy rats were randomly divided into sham-operative group, focal cerebral isehemia group, ginsenoside Rglgroups （low, medium and high concentrations） and drug control group. Rats were intraperitoneally injected saline 45 mg/kg, saline 45 mg/kg＋ginsenosides Rgl 10, 20 and 40 mg/kg, nimodipine 1 mg/kg 5 d before surgery, respectively. Focal cerebral isch- emia model was made by middle cerebral artery occluding in rats. The neurological deficit score and TTC staining were used to verify the success of the rat model. The expressions of PARP-1 and TNFR1 were evaluated by immunohistoehemical meth- od and Western blot technique. Results There were obvious symptoms of neurological deficit and large pale infarct area in focal cerebral ischemia group compared with those of sham-operative group.
ISSN:0253-9896
DOI:10.3969/j.issn.0253-9896.2015.03.006