双三元液相色谱-串联电喷雾检测器用于中药注射剂大分子物质筛查研究

A size exclusion chromatography method ( SEC) was established for the screening of macromolecules in traditional Chinese medicine (TCM) injections. In the experiment, TCM was collected through 0. 45μm hybrid filter and separated by SEC with MAbPac SEC-1 column. Acetonitrile was added by post-column...

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Bibliographic Details
Published in分析化学 Vol. 42; no. 12; pp. 1871 - 1872
Main Author 张婷婷 王玉 谷丹华 张俐 金燕
Format Journal Article
LanguageChinese
Published 赛默飞世尔科技色谱产品应用研究中心,上海,200100%中国药科大学，南京210009 2014
江苏省食品药品检验所，南京210008%中国药科大学,南京,210009%江苏省食品药品检验所,南京,210008
Subjects
三元
中药注射剂
串联
大分子物质
检测器
液相色谱
电喷雾
筛查
Size exclusion chromatography
Liquid chromatography
Macromolecule screening
Charged aerosol detection
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ISSN0253-3820
DOI10.11895/j.issn.0253-3820.140849

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Summary:A size exclusion chromatography method ( SEC) was established for the screening of macromolecules in traditional Chinese medicine (TCM) injections. In the experiment, TCM was collected through 0. 45μm hybrid filter and separated by SEC with MAbPac SEC-1 column. Acetonitrile was added by post-column mode with a flow rate of 0. 2 mL/min to enhance the detection sensitivity, and 25 mmol/L ammonium formate was used as the mobile phase at flow rate of 0. 2 mL/min. The charged aerosol detection was carried out with Dextran and Tween as the reference standards. The molecular weight distribution of Dextran ranged from 2500 to 133800 had a good linear relationship with the retention time. No macromolecules were detected out in the selected traditional Chinese medicine injections except Kuhuang injections.
Bibliography:ZHANG Ting-Ting , WANG Yu , GU Dan-Hua , ZHANG Li , JIN Yan 1（Shanghai Application Lab, Thermofisher Scierttific, Shanghai 200100, China） 2（ China Pharmaceutical University, Nanjin 210009, China） 3 （ Jiangsu Institute for Food and Drug Control, Nanfin 210009, China）
Charged aerosol detection; Liquid chromatography; Size exclusion chromatography; Macromolecule screening
22-1125/O6
A size exclusion chromatography method （SEC） was established for the screening of macromolecules in traditional Chinese medicine （TCM） injections. In the experiment, TCM was collected through 0.45 Ixm hybrid filter and separated by SEC with MAbPac SEC-1 column. Acetonitrile was added by post-column mode with a flow rate of 0.2 mL/min to enhance the detection sensitivity, and 25 mmol/L ammonium formate was used as the mobile phase at flow rate of 0.2 mL/min. The charged aerosol detection was carried out with Dextran and Tween as the reference standards. The molecular weight distribution of Dextran ranged from 2500 to 133800 had a good li
ISSN:0253-3820
DOI:10.11895/j.issn.0253-3820.140849