积雪草漆斑病病原鉴定及室内药剂筛选

近年来,在广西各地积雪草种植基地,出现一种为害极重的病害,该病害病原菌主要为害积雪草叶片、叶柄,可致使叶片大面积枯死,该病害发生普遍、侵染期长,严重影响积雪草药材的品质和产量。该研究采用常规组织分离法从积雪草病叶上分离病原菌并根据柯赫氏法则验证其致病性;通过菌落特征、菌株形态及ITS序列分析鉴定病原菌;在室内利用生长速率法测定10种杀菌剂对该菌的抑制活性。结果表明：为害积雪草叶片的病原菌为泛生漆斑菌（Myrothecium inundatum）;该菌侵染性强,接种至健康积雪草叶片后24 h即可发病,并迅速扩展引起叶片萎蔫;杀菌剂60%唑醚·代森联水分散粒剂、45%恶霉灵·甲霜可湿性粉剂、1.8...

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Bibliographic Details
Published in广西植物 Vol. 36; no. 8; pp. 993 - 999
Main Author 胡凤云 蒋妮 叶云峰 蒋水元
Format Journal Article
LanguageChinese
Published 广西药用植物园,南宁,530023%广西植物功能物质研究与利用重点实验室，广西壮族自治区中国科学院 广西植物研究所，广西 桂林541600 2016
Subjects
泛生漆斑菌
病原菌
积雪草
药剂筛选
积雪草
药剂筛选
病原菌
Centella asiatica
pathogen
Myrothecium inundatum
泛生漆斑菌
screening of fungicides
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ISSN1000-3142
DOI10.11931/guihaia.gxzw201410047

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Summary:近年来,在广西各地积雪草种植基地,出现一种为害极重的病害,该病害病原菌主要为害积雪草叶片、叶柄,可致使叶片大面积枯死,该病害发生普遍、侵染期长,严重影响积雪草药材的品质和产量。该研究采用常规组织分离法从积雪草病叶上分离病原菌并根据柯赫氏法则验证其致病性;通过菌落特征、菌株形态及ITS序列分析鉴定病原菌;在室内利用生长速率法测定10种杀菌剂对该菌的抑制活性。结果表明：为害积雪草叶片的病原菌为泛生漆斑菌（Myrothecium inundatum）;该菌侵染性强,接种至健康积雪草叶片后24 h即可发病,并迅速扩展引起叶片萎蔫;杀菌剂60%唑醚·代森联水分散粒剂、45%恶霉灵·甲霜可湿性粉剂、1.8%辛菌胺醋酸盐水剂、80%代森锰锌可湿性粉剂室内对积雪草漆斑病病原菌的抑菌率均在98%以上,抑菌效果极强,建议在生产中轮换应用防治积雪草漆斑病。15%咪鲜胺微乳剂、70%甲基硫菌灵可湿性粉剂和10%苯醚菌酯悬浮剂对该病原菌的抑菌率也均在92%以上,抑菌效果良好,在生产中可酌情考虑使用。
Bibliography:Centella asiatica; Myrothecium inundatum; pathogen; screening of fungicides
45-1134/Q
In recent years,a new disease of Centella asiatica occurred seriously in producing area of Guangxi,this pathogen usually damages the leaves and petioles,and it can lead a large area of C. asiatica to wither. It occured very common and had a long infection period on C. asiatica,so the yield and quality of C. asiatica declined seriously. We used general tissue separation to isolate from infected plants,and corroborated the pathogenicity according to Koch＇s Rule.; and identified the pathogen mainly based on the morphological characters and molecular analysis of internal transcribed spacer（ ITS） sequences,using growth rate method,antimicrobial activity of ten fungicides assayed against the pathogen. The result showed that the pathogen Myrothecium inundatum was discovered,which resulted in black spot disease of Centella asiatica. It had strong infectiousness and occurred on healthy leaves in 24 h,and expanded rapidly to bring about
ISSN:1000-3142
DOI:10.11931/guihaia.gxzw201410047