高山离子芥试管苗在PEG-6000模拟干旱条件下的生理响应

以高山离子芥（Chorispora bungeana）为试材,采用固液培养法,设置对照（不添加PEG-6000,CK）、5%PEG-6000、10%PEG-6000、20%PEG-6000、40%PEG-6000五个干旱处理水平,研究了不同浓度PEG-6000模拟干旱环境下,对高山离子芥幼苗生理生化特性的影响。结果表明：干旱胁迫下,随着不同的处理时间,脯氨酸含量始终保持上升的趋势且含量显著高于对照;可溶性蛋白的含量也有波动,呈现先上升后下降的趋势;丙二醛（MDA）含量,随着时间的延长,呈现先升高再下降的趋势且含量显著高于对照。幼苗叶片叶绿素总含量呈现先升高后下降的趋势,在浓度为40%PEG-6...

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Bibliographic Details
Published in广西植物 Vol. 35; no. 1; pp. 77 - 83
Main Author 杨宁 王程亮 李宜珅 王新霞 陈霞 牛涛
Format Journal Article
LanguageChinese
Published 西北师范大学 生命科学学院,兰州,730070 2015
Subjects
PEG-6000
干旱胁迫
渗透调节
生理指标
高山离子芥
PEG-6000
osmoregulation
drought stress
干旱胁迫
高山离子芥
生理指标
Chorispora bungeana
渗透调节
physiological indicators
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ISSN1000-3142
DOI10.11931/guihaia.gxzw201404044

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Summary:以高山离子芥（Chorispora bungeana）为试材,采用固液培养法,设置对照（不添加PEG-6000,CK）、5%PEG-6000、10%PEG-6000、20%PEG-6000、40%PEG-6000五个干旱处理水平,研究了不同浓度PEG-6000模拟干旱环境下,对高山离子芥幼苗生理生化特性的影响。结果表明：干旱胁迫下,随着不同的处理时间,脯氨酸含量始终保持上升的趋势且含量显著高于对照;可溶性蛋白的含量也有波动,呈现先上升后下降的趋势;丙二醛（MDA）含量,随着时间的延长,呈现先升高再下降的趋势且含量显著高于对照。幼苗叶片叶绿素总含量呈现先升高后下降的趋势,在浓度为40%PEG-6000胁迫下,叶绿素总含量均显著低于对照;叶绿素a则有显著降低的趋势;叶绿素b在浓度为5%PEG-6000胁迫下显著升高,在浓度为10%、20%、40%PEG-6000胁迫下也呈现显著升高的趋势;但其含量总体低于叶绿素a;Chla/Chlb的值经历了先升高后下降的趋势且均高于对照。在各浓度及不同时间处理胁迫下,高山离子芥叶片K＋、Na^＋、Ca^2＋、Mg2＋含量有明显波动：K^＋、Ca2在5%和10%PEG-6000胁迫6、12、24、48、72h后均显著高于对照,而40%PEG-6000胁迫后均显著低于对照。Na^＋在5%PEG-6000各时间段胁迫后含量较对照有所增加,在其它浓度和时间处理下其含量均低于对照;而Mg^2＋含量在40%PEG-6000胁迫下虽有下降趋势但均显著高于对照。表明在干旱胁迫下,高山离子芥通过改变渗透调节物、光合系统中叶绿素含量及离子含量等,启动应对外界干旱环境的耐旱响应机制,从生理角度揭示了高山离子芥响应耐旱的生理生态机理。
Bibliography:Chorispora bungeanaia a perennial herb,which grows in Qinhai-Tibet Plateau,where has a elevation of2 600-3 700 m,and has the features of less rain,drought,low temperature,high ambient ultraviolet radiation.The special growing environment gives it the unique molecular and physiological resistance to stress response mechanism.Recently,there are many reports about the molecular and physiological resistance to the low temperature stress response mechanism.However,it has not been reported about what physiological ecology strategies it has in the process of long-term growth to adapt to drought environment.In this study,Taking C.bungeanaplantlets in vitro as material,the current study was carried out to investigate the effect of drought stress on a Qinhai-Tibet Plateau plant C.bungeana with solid-liquid culture medium.There were five drought levels being set：the control（without adding PEG-6000,CK）,mild drought stress（5% PEG-6000）,moderate drought stress（10% and 20% PEG-6000）,and severe drought stress（40% PEG-6000）an
ISSN:1000-3142
DOI:10.11931/guihaia.gxzw201404044