测定植物胞内游离钠离子的研究进展

植物耐盐机制的研究一直是植物抗性研究的焦点。近年来,随着生物学不断发展和新荧光标记技术的运用,胞内钠离子测定逐渐应用于植物盐胁迫研究中。该文论述了以下三方面问题：（1）分别介绍了 SBFI、Sodium Green 和 CoroNa Green 三种钠离子荧光指示剂：SBFI 是一种双激发波长指示剂,其激发波长是340 nm/380 nm,发射波长是500 nm；Sodium Green 和 CoroNa Green 是单波长指示剂,其激发波长分别是507 nm和492 nm,发射波长分别是532 nm 和516 nm；（2）比较了酯导入、酸导入、电穿孔和显微注射等几种常见荧光指示剂载入胞内方...

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Bibliographic Details
Published in广西植物 Vol. 35; no. 3; pp. 442 - 446
Main Author 刘瑞娟 蔡振媛 车国冬
Format Journal Article
LanguageChinese
Published 中国科学院高原生物适应与进化重点实验室,西宁,810001%中国科学院西北高原生物研究所,西宁,810001 2015
Subjects
Na
低温酯导入
植物
离子浓度计算
细胞内游离
荧光指示剂
ester loading under low temperature
植物
低温酯导入
fluorescent indicators
细胞内游离 Na+
intracellular free sodium
荧光指示剂
plants
离子浓度计算
calculating the ion concentration
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ISSN1000-3142
DOI10.11931/guihaia.gxzw201404034

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Summary:植物耐盐机制的研究一直是植物抗性研究的焦点。近年来,随着生物学不断发展和新荧光标记技术的运用,胞内钠离子测定逐渐应用于植物盐胁迫研究中。该文论述了以下三方面问题：（1）分别介绍了 SBFI、Sodium Green 和 CoroNa Green 三种钠离子荧光指示剂：SBFI 是一种双激发波长指示剂,其激发波长是340 nm/380 nm,发射波长是500 nm；Sodium Green 和 CoroNa Green 是单波长指示剂,其激发波长分别是507 nm和492 nm,发射波长分别是532 nm 和516 nm；（2）比较了酯导入、酸导入、电穿孔和显微注射等几种常见荧光指示剂载入胞内方法的优缺点,重点介绍了一种无损伤低温抑制酯酶法：先将荧光指示剂在缓冲液中低温（4℃）处理2 h,随后回到常温（20℃）在不含荧光指示剂的缓冲液中孵育2 h；（3）阐述了胞内离子浓度计算公式,包括单波长测定公式、双波长比率测定公式。
Bibliography:45-1134/Q
LIU Rui-Juan, CAI Zhen-Yuan, CHE Guo-Dong （ 1. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China; 2. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China ）
intracellular free sodium;plants;fluorescent indicators;ester loading under low temperature;calculating the ion concentration
The mechanism of salt tolerance in plants had been the focus of plant resistance research in the past years.As the development of biology and application of new fluorescent labeling technologies,determination of intra-cellular free sodium had been gradually applied to the study of salt tolerance in plants.This review discussed three points as follows：（1）Introduction of three fluorescent indicators of intracellular free sodium：SBFI,Sodium Green and CoroNa Green.SBFI was a kind of fluorescent indicator for excitation ratio measurements,its emission ratio de-tected at 500 nm when excited at 34
ISSN:1000-3142
DOI:10.11931/guihaia.gxzw201404034