激光剥蚀电感耦合等离子体质谱法中生物样品的元素分馏效应研究

采用213 nm-纳秒激光剥蚀系统对生物基体样品的剥蚀颗粒进行研究,优化了激光剥蚀条件。在剥蚀能量为25%,束斑直径为200μm,剥蚀速率为20μm/s,频率为20 Hz,载气为700 m L He＋700 m L Ar时,信号强度及稳定性最佳。以31P为内标元素,最佳剥蚀条件下,考察了56个元素的相对分馏因子。结果表明,生物基体的剥蚀颗粒相较于NIST 610玻璃标样更大,达到3μm;生物基体中元素分馏效应相较于玻璃基体小,大多数元素的相对分馏因子达到1.0±0.1。探讨了生物基体中元素分馏机理,分析了生物基体相较于玻璃基体剥蚀颗粒大,而相对分馏因子未明显增大的原因。一方面可能是粒径3μm的...

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Bibliographic Details
Published in分析化学 Vol. 45; no. 6; pp. 868 - 873
Main Author 李青 张国霞 陈奕睿 汪正 丁传贤
Format Journal Article
LanguageChinese
Published 中国科学院大学, 北京 100049%中国科学院上海硅酸盐研究所,上海,200050 2017
中国科学院上海硅酸盐研究所, 上海 200050
Subjects
分馏效应
激光剥蚀-电感耦合等离子体质谱
生物样品
纳秒激光
激光剥蚀-电感耦合等离子体质谱
分馏效应
生物样品
Nanosecond laser
Biological sample
纳秒激光
Laser ablation-inductively coupled plasma-mass spectrometry
Fractionation effect
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ISSN0253-3820
DOI10.11895/j.issn.0253-3820.170052

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Summary:采用213 nm-纳秒激光剥蚀系统对生物基体样品的剥蚀颗粒进行研究,优化了激光剥蚀条件。在剥蚀能量为25%,束斑直径为200μm,剥蚀速率为20μm/s,频率为20 Hz,载气为700 m L He＋700 m L Ar时,信号强度及稳定性最佳。以31P为内标元素,最佳剥蚀条件下,考察了56个元素的相对分馏因子。结果表明,生物基体的剥蚀颗粒相较于NIST 610玻璃标样更大,达到3μm;生物基体中元素分馏效应相较于玻璃基体小,大多数元素的相对分馏因子达到1.0±0.1。探讨了生物基体中元素分馏机理,分析了生物基体相较于玻璃基体剥蚀颗粒大,而相对分馏因子未明显增大的原因。一方面可能是粒径3μm的颗粒进入电感耦合等离子体后能原子化;另一方面,大的剥蚀颗粒的富集效应相对较小。进一步对分馏效应的影响因素进行研究,发现分馏效应与激光剥蚀能量、激光频率和扫描速率相关,并且与元素的氧化物沸点负相关,与氧化物键能和电离能正相关。
Bibliography:LI Qing1,2,ZHANG Guo-Xia1,CHEN Yi-Rui1,WANG Zheng1,DING Chuan-Xian1 1 （ Shanghai Institute sf Cerapic,Chinese Acadepy of Sciences, Shanghai 200050,China）2（ University sf Chinese Acadepy of Sciences,Beijing 100049,China）
22-1125/O6
The ablated aerosols of biological matrix sample were studied using 213 nm nanosecond laser ablation system. The stable signal intensity and high sensitivity were obtained when the laser energy was 25 %, the spot size was 200 μm,the scan rate was 20 μm/s,the frequency was 20 Hz and th e carrier gas was 700 mL He ＋ 700 mL Ar. Relative fractionation index of 56 elements were investigated and 31P as the internal standard element was selected under the optimized laser ablation conditions. The results showed that particle size of the biological sample was 3 μm, which was larger compared with NIST 610 sample . Element fractionation in biological sample was smaller than in glass sample , and relative fractionation index of most elements attained 1.0 土 0. 1. Element fractionation mechanism of
ISSN:0253-3820
DOI:10.11895/j.issn.0253-3820.170052