新型三维复合仿生网络的构建及其组织相容性评价
目的构建并评价新型三维复合仿生网络的组织相容性。方法采用仿生学方法，将壳聚糖、羟基磷灰石、明胶、果胶按一定比例制成新型三维复合仿生网络，将小鼠胚胎成骨细胞MC3T3－E1与材料进行复合培养，通过倒置相差显微镜、石蜡切片常规染色、扫描电镜、F－DA荧光染色法评价细胞相容性；将制备好的生物支架材料植入SD大鼠的背部皮下，术后2、4、8、12周评价组织相容性、血管化能力及体内降解情况。结果新型三维复合仿生网络呈三维多孔状，细胞在材料上贴附生长良好，呈多角形或梭形，形态饱满；皮下包埋实验发现：早期有轻微的炎症反应，随时间延长而消退，后期有血管化发生，材料降解吸收比较缓慢。结论新型三维复合仿生网络组织相...
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| Published in | 天津医药 Vol. 43; no. 3; pp. 233 - 236 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
河北联合大学基础医学院病理教研室 邮编063000%天津大学化工学院
2015
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-9896 |
| DOI | 10.3969/j.issn.0253-9896.2015.03.003 |
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| Abstract | 目的构建并评价新型三维复合仿生网络的组织相容性。方法采用仿生学方法，将壳聚糖、羟基磷灰石、明胶、果胶按一定比例制成新型三维复合仿生网络，将小鼠胚胎成骨细胞MC3T3－E1与材料进行复合培养，通过倒置相差显微镜、石蜡切片常规染色、扫描电镜、F－DA荧光染色法评价细胞相容性；将制备好的生物支架材料植入SD大鼠的背部皮下，术后2、4、8、12周评价组织相容性、血管化能力及体内降解情况。结果新型三维复合仿生网络呈三维多孔状，细胞在材料上贴附生长良好，呈多角形或梭形，形态饱满；皮下包埋实验发现：早期有轻微的炎症反应，随时间延长而消退，后期有血管化发生，材料降解吸收比较缓慢。结论新型三维复合仿生网络组织相容性好，易于血管化，是一种很好的骨组织工程支架材料。 |
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| AbstractList | R318.08; 目的：构建并评价新型三维复合仿生网络的组织相容性。方法采用仿生学方法，将壳聚糖、羟基磷灰石、明胶、果胶按一定比例制成新型三维复合仿生网络，将小鼠胚胎成骨细胞MC3T3-E1与材料进行复合培养，通过倒置相差显微镜、石蜡切片常规染色、扫描电镜、F-DA荧光染色法评价细胞相容性；将制备好的生物支架材料植入SD大鼠的背部皮下，术后2、4、8、12周评价组织相容性、血管化能力及体内降解情况。结果新型三维复合仿生网络呈三维多孔状，细胞在材料上贴附生长良好，呈多角形或梭形，形态饱满；皮下包埋实验发现：早期有轻微的炎症反应，随时间延长而消退，后期有血管化发生，材料降解吸收比较缓慢。结论新型三维复合仿生网络组织相容性好，易于血管化，是一种很好的骨组织工程支架材料。 目的构建并评价新型三维复合仿生网络的组织相容性。方法采用仿生学方法，将壳聚糖、羟基磷灰石、明胶、果胶按一定比例制成新型三维复合仿生网络，将小鼠胚胎成骨细胞MC3T3－E1与材料进行复合培养，通过倒置相差显微镜、石蜡切片常规染色、扫描电镜、F－DA荧光染色法评价细胞相容性；将制备好的生物支架材料植入SD大鼠的背部皮下，术后2、4、8、12周评价组织相容性、血管化能力及体内降解情况。结果新型三维复合仿生网络呈三维多孔状，细胞在材料上贴附生长良好，呈多角形或梭形，形态饱满；皮下包埋实验发现：早期有轻微的炎症反应，随时间延长而消退，后期有血管化发生，材料降解吸收比较缓慢。结论新型三维复合仿生网络组织相容性好，易于血管化，是一种很好的骨组织工程支架材料。 |
| Abstract_FL | Objective To construct novel 3-D composite bionic network and evaluate the histocompatibility . Meth?ods The novel 3-D composite bionic network was prepared from chitosan, hydroxyapatite, gelatin and pectin in certain ra?tio by biomimtic approach, which was co-cultured with MC3T3-E1. The cell compatibility was studied by using inverted phase contrast microscope, routine paraffin section staining, scanning electron microscopy and F-DA staining. The resultant scaffold material was implanted into the dorsal subcutaneous space of SD rats. The histocompatibility, blood vessel capabili?ties and the degradation of the material were observed 2, 4, 8 and 12 weeks after surgery. Results The structure of novel 3-D composite bionic network was three-dimensional and porous. The cells attached on scaffolds attached and grew well with polygonal or fusiform form. It was found that inflammatory reactions were alleviated gradually in the early stage . There was an increasing angiogenesis at late stage. Materials degraded and absorbed more slowly. Conclusion The present study sug?gests that the novel 3-D composite bionic network has good histocompatibility with easy vascularization, and will be a candi?date scaffold for bone tissue engineering. |
| Author | 崔曼 张欣 马丽桃 熊艳杰 车鹏程 姚芳莲 孙红 |
| AuthorAffiliation | 河北联合大学基础医学院病理教研室,063000 天津大学化工学院 |
| AuthorAffiliation_xml | – name: 河北联合大学基础医学院病理教研室 邮编063000%天津大学化工学院 |
| Author_FL | CUI Man ZHANG Xin MA Litao XiongYanjie SUN Hong CHE Pengcheng YAO Fanglian |
| Author_FL_xml | – sequence: 1 fullname: CUI Man – sequence: 2 fullname: ZHANG Xin – sequence: 3 fullname: MA Litao – sequence: 4 fullname: XiongYanjie – sequence: 5 fullname: CHE Pengcheng – sequence: 6 fullname: YAO Fanglian – sequence: 7 fullname: SUN Hong |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| Keywords | chitosan 壳聚糖 tissue engineering 3-D composite bionic net-work 组织相容性 neovascularization,physiologic 三维复合仿生网络 histocompatibility 新生血管化,生理性 组织工程 |
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| Notes | neovascularization, physiologic ; histocompatibility ; tissue engineering; chitosan ; 3-D composite bionic net-work CUI Man, ZHANG Xin, MA Litao, XiongYanjie, CHE Pengcheng, YAO Fanglian, SUN Hong（ 1 Department of Pathology, Basic Medical Sciences, Hebei United University, Tangshan 063000, China ; 2 School of Chemical Engineering and Technology, Tianjin University） Objective To construct novel 3-D composite bionic network and evaluate the histocompatibility . Meth- ods The novel 3-D composite bionic network was prepared from chitosan, hydroxyapatite, gelatin and pectin in certain ra- tio by biomimtic approach, which was co-cultured with MC3T3-E1. The cell compatibility was studied by using inverted phase contrast microscope, routine paraffin section staining, scanning electron microscopy and F-DA staining. The resultant scaffold material was implanted into the dorsal subcutaneous space of SD rats. The histocompatibility, blood vessel capabili- ties and the degradation of the material were observed 2, 4, 8 and 1 |
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| Publisher | 河北联合大学基础医学院病理教研室 邮编063000%天津大学化工学院 |
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| SubjectTerms | 三维复合仿生网络 壳聚糖 新生血管化 生理性 组织工程 组织相容性 |
| Title | 新型三维复合仿生网络的构建及其组织相容性评价 |
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