新型三维复合仿生网络的构建及其组织相容性评价

目的构建并评价新型三维复合仿生网络的组织相容性。方法采用仿生学方法，将壳聚糖、羟基磷灰石、明胶、果胶按一定比例制成新型三维复合仿生网络，将小鼠胚胎成骨细胞MC3T3－E1与材料进行复合培养，通过倒置相差显微镜、石蜡切片常规染色、扫描电镜、F－DA荧光染色法评价细胞相容性；将制备好的生物支架材料植入SD大鼠的背部皮下，术后2、4、8、12周评价组织相容性、血管化能力及体内降解情况。结果新型三维复合仿生网络呈三维多孔状，细胞在材料上贴附生长良好，呈多角形或梭形，形态饱满；皮下包埋实验发现：早期有轻微的炎症反应，随时间延长而消退，后期有血管化发生，材料降解吸收比较缓慢。结论新型三维复合仿生网络组织相...

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Bibliographic Details
Published in天津医药 Vol. 43; no. 3; pp. 233 - 236
Main Author 崔曼 张欣 马丽桃 熊艳杰 车鹏程 姚芳莲 孙红
Format Journal Article
LanguageChinese
Published 河北联合大学基础医学院病理教研室 邮编063000%天津大学化工学院 2015
Subjects
三维复合仿生网络
壳聚糖
新生血管化
生理性
组织工程
组织相容性
chitosan
壳聚糖
tissue engineering
3-D composite bionic net-work
组织相容性
neovascularization,physiologic
三维复合仿生网络
histocompatibility
新生血管化,生理性
组织工程
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ISSN0253-9896
DOI10.3969/j.issn.0253-9896.2015.03.003

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Summary:目的构建并评价新型三维复合仿生网络的组织相容性。方法采用仿生学方法，将壳聚糖、羟基磷灰石、明胶、果胶按一定比例制成新型三维复合仿生网络，将小鼠胚胎成骨细胞MC3T3－E1与材料进行复合培养，通过倒置相差显微镜、石蜡切片常规染色、扫描电镜、F－DA荧光染色法评价细胞相容性；将制备好的生物支架材料植入SD大鼠的背部皮下，术后2、4、8、12周评价组织相容性、血管化能力及体内降解情况。结果新型三维复合仿生网络呈三维多孔状，细胞在材料上贴附生长良好，呈多角形或梭形，形态饱满；皮下包埋实验发现：早期有轻微的炎症反应，随时间延长而消退，后期有血管化发生，材料降解吸收比较缓慢。结论新型三维复合仿生网络组织相容性好，易于血管化，是一种很好的骨组织工程支架材料。
Bibliography:neovascularization, physiologic ; histocompatibility ; tissue engineering; chitosan ; 3-D composite bionic net-work
CUI Man, ZHANG Xin, MA Litao, XiongYanjie, CHE Pengcheng, YAO Fanglian, SUN Hong（ 1 Department of Pathology, Basic Medical Sciences, Hebei United University, Tangshan 063000, China ; 2 School of Chemical Engineering and Technology, Tianjin University）
Objective To construct novel 3-D composite bionic network and evaluate the histocompatibility . Meth- ods The novel 3-D composite bionic network was prepared from chitosan, hydroxyapatite, gelatin and pectin in certain ra- tio by biomimtic approach, which was co-cultured with MC3T3-E1. The cell compatibility was studied by using inverted phase contrast microscope, routine paraffin section staining, scanning electron microscopy and F-DA staining. The resultant scaffold material was implanted into the dorsal subcutaneous space of SD rats. The histocompatibility, blood vessel capabili- ties and the degradation of the material were observed 2, 4, 8 and 1
ISSN:0253-9896
DOI:10.3969/j.issn.0253-9896.2015.03.003