聚合物/磷酸盐双水相分离乳清分离蛋白中α-乳白蛋白和β-乳球蛋白条件的优化
建立了基于聚（乙二醇-ran-丙二醇）单丁基醚（聚合物）与磷酸盐、氯化钠的双水相体系对乳清分离蛋白（WPI）中的α-乳白蛋白（α-LA）和β-乳球蛋白（β-LG）进行分离,并对其分离条件进行了优化。系统地研究了双水相体系p H值、聚合物与KH_2PO_4溶液体积比、Na Cl添加量和WPI浓度对α-LA和β-LG的分离效果的影响,采用液相色谱法对α-LA和β-LG的分离效果进行评价。结果表明,聚合物/KH2PO4双水相体系p H=4.0,40%（m/m）聚合物与15.5%（m/m）KH2PO4的体积比为4 m L∶4 m L,Na Cl添加量为0.40 g/10 m L,WPI浓度为1 mg/...
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| Published in | 分析化学 Vol. 44; no. 5; pp. 754 - 759 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
东北农业大学理学院应用化学系,哈尔滨,150030
2016
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.11895/j.issn.0253-3820.150800 |
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| Summary: | 建立了基于聚（乙二醇-ran-丙二醇）单丁基醚（聚合物）与磷酸盐、氯化钠的双水相体系对乳清分离蛋白（WPI）中的α-乳白蛋白（α-LA）和β-乳球蛋白（β-LG）进行分离,并对其分离条件进行了优化。系统地研究了双水相体系p H值、聚合物与KH_2PO_4溶液体积比、Na Cl添加量和WPI浓度对α-LA和β-LG的分离效果的影响,采用液相色谱法对α-LA和β-LG的分离效果进行评价。结果表明,聚合物/KH2PO4双水相体系p H=4.0,40%（m/m）聚合物与15.5%（m/m）KH2PO4的体积比为4 m L∶4 m L,Na Cl添加量为0.40 g/10 m L,WPI浓度为1 mg/m L时,α-LA和β-LG分离效果最好,上相中α-LA的萃取率为98.2%,下相中β-LG的萃取率为96.6%。 |
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| Bibliography: | ZHANG He, JIANG Bin, FENG Zhi-Biao , Qu Yu-Xiao, LI Xuan （ Department of Applied Chemistry, Northeast Agricultural University, Harbin 150030, China） Aqueous two-phase system; Whey protein isolate; Poly （ethylene glycol-ran-propylene glycol） monobutyl ether; Separation 22-1125/O6 Poly （ethylene glycol-ran-propylene glycol） monobutyl ether/phosphate aqueous two-phase system （ATPS） coupled with high performance liquid chromatography （HPLC） was developed for the separation of α-lactalbumin （α-LA） and β-1actoglobulin （β-LG） in whey protein isolate （WPI）. The main parameters relating to this method were investigated and optimized. The effects of pH value, volume ratio of polymer and KH2PQ solutions, concentration of NaC1 and WPI on the partition of α-LA and β-LG in WPI were investigated. The results showed that efficient separation of both proteins in WPI could be achieved by using the ATPS with 4 mL poly （ethylene glycol-ran-propylene glycol） monobutyl ether solution （40%, m/m） and 4 mL KH2PO4 solution （ 15.5%, m/m）, |
| ISSN: | 0253-3820 |
| DOI: | 10.11895/j.issn.0253-3820.150800 |