快速测定含水溶液中乙醇的氧同位素比值

近年来,乙醇的氧同位素比值(δ^18O)在果汁和饮料酒真实性鉴别中起着重要作用,利用该指标可检测产品中的外源水、追溯产品的产地。本工作采用多孔聚合物气相色谱柱实现了水与乙醇的在线快速分离,建立了溶剂稀释后直接用气相色谱-裂解-稳定同位素比值质谱(GC-TC-IRMS)测定溶液中乙醇δ^18O值的方法。实验结果表明,该方法可排除水对乙醇δ^18O分析的干扰,乙醇浓度在1%~100%时测定稳定性良好,在不同水溶液中乙醇δ^18O的测定值保持一致;乙醇δ^18O值重复性和再现性的标准偏差均优于0.5‰,并通过欧盟实验室间能力验证(FIT-PTS)证明了方法的准确性。该方法具有样品用量少(仅需70~2...

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Bibliographic Details
Published in质谱学报 Vol. 37; no. 5; pp. 471 - 480
Main Author 钟其顶 王道兵 李国辉
Format Journal Article
LanguageChinese
Published 全国食品发酵标准中心,北京100015 2016
中国食品发酵工业研究院,北京100015
Subjects
Online AccessGet full text
ISSN1004-2997
DOI10.7538/zpxb.youxian.2016.0033

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Summary:近年来,乙醇的氧同位素比值(δ^18O)在果汁和饮料酒真实性鉴别中起着重要作用,利用该指标可检测产品中的外源水、追溯产品的产地。本工作采用多孔聚合物气相色谱柱实现了水与乙醇的在线快速分离,建立了溶剂稀释后直接用气相色谱-裂解-稳定同位素比值质谱(GC-TC-IRMS)测定溶液中乙醇δ^18O值的方法。实验结果表明,该方法可排除水对乙醇δ^18O分析的干扰,乙醇浓度在1%~100%时测定稳定性良好,在不同水溶液中乙醇δ^18O的测定值保持一致;乙醇δ^18O值重复性和再现性的标准偏差均优于0.5‰,并通过欧盟实验室间能力验证(FIT-PTS)证明了方法的准确性。该方法具有样品用量少(仅需70~200μL)、分析速度快(约18min)、操作简单方便等特点,可为乙醇δ^18O在果汁和饮料酒真实性领域的研究与应用提供方法参考。
Bibliography:ZHONG Qi-ding1,2 ,WANG Dao -bing 1,2,LI Guo -hui1,2 (1. China National Research Institute of Food and Fermentation Industries ?, Beijing 100015, Chinas 2. National Standardization Center of Food&Fermentation Industry , Beijing 100015, China)
11-2979/TH
ethanol; stable oxygen isotoperatio; aqueous samples; e xtraction
During thelast years , it has been demons t rated that δ^18O isotope an aly sis of ethanol in juices and alcoholic beverages provides powerful information to assess their adulteration by addition of water, or to authenticate their geographical origin. Howev-er, it is difficult to accurately and conveniently quantify it due to the measurement prin-ciple and its water miscibility. To eliminate the impact of water on ethanol δ^18O analy-sis ,a porous-polymer-bonded GC column was used to achieve a baseline separation of ethanol and water, then the water was vented by backflush function and the ethanol was converted and analyzed. This is the first manuscript presenting a systematic evaluation and charact
ISSN:1004-2997
DOI:10.7538/zpxb.youxian.2016.0033