Expression of ASFV p17 in CHO cells and identification of one novel epitope using a monoclonal antibody

• Published in: Virus research Vol. 336; p. 199194
• Main Authors: Li, Liwei, Qiao, Sina, Wang, Shumao, Liu, Jiachen, Zhao, Kuan, Zhou, Yanjun, Li, Guoxin, Jiang, Yifeng, Liu, Changlong, Tong, Guangzhi, Tong, Wu, Gao, Fei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.10.2023; Elsevier
• Subjects: African swine fever; African swine fever virus; ASFV; CHO cells; Epitope; epitopes; genotype; monoclonal antibodies; Monoclonal antibody; P17; porcine reproductive and respiratory syndrome; vaccines; viruses; Monoclonal antibody; Epitope; ASFV; CHO cells; P17; epitope; monoclonal antibody; p17
• ISSN: 0168-1702; 1872-7492; 1872-7492
• DOI: 10.1016/j.virusres.2023.199194

•ASFV p17 was successfully expressed in CHO cells using a suspension culture system.•1B4 recognized a conservative linear epitope (8LLSHNLSTREGIK20) among genotype i and genotype II ASFV strains.•1B4 effectively recognized the ectopically expressed p17 and foreign gene expression in recombinant PRRSV expressing ASFV p17.•1B4 can be a useful tool to evaluate the expression of p17 in the recombinant virus, making it meaningful for future vaccine research on ASF. As a highly pathogenic large DNA virus, African swine fever virus (ASFV) has huge particles and numerous encoded proteins. At present, few of the existing studies on ASFV proteins have investigated the function of p17. Specific antibodies against p17 to promote the development of prevention techniques against African swine fever (ASF) are urgently needed. Herein, we successfully expressed ASFV p17 in CHO cells using a suspension culture system and generated a monoclonal antibody (mAb) against p17. The mAb recognized a novel linear epitope (8LLSHNLSTREGIK20) and exhibited specific reactivity, which was conducive to the identification of ectopically expressed p17, the recombinant porcine reproductive and respiratory syndrome virus expressing p17, and the ASFV-SY18. The epitope was conservative among genotype I and genotype II ASFV strains. Overall, the mAb against p17 revealed efficient detection and promising application prospects, making it a useful tool for future vaccine research on ASF. Determination of the conserved linear epitope of p17 would contribute to the in-depth exploration of the biological function of ASFV antigen protein.