斜带石斑鱼（♀）×鞍带石斑鱼（♂）杂交子代幼鱼消化道粘液细胞和胃泌素细胞的研究
利用组织化学（AB-PAS）和免疫组织化学（SABC）的方法分别对斜带石斑鱼（Epinephelus coioides♀）×鞍带石斑鱼（E.lanceolatus♂）杂交子代（青龙斑）幼鱼消化道的粘液细胞和胃泌素（gastrin,Gas）分泌细胞的分布进行系统的研究。青龙斑幼鱼食道中有Ⅰ、Ⅱ、Ⅲ和Ⅳ型粘液细胞,含中性粘多糖和酸性粘多糖。贲门胃粘液细胞有Ⅰ、Ⅱ、Ⅲ种类型,胃体部有Ⅰ、Ⅲ、Ⅳ型粘液细胞,在胃腺的周围含有较多的Ⅰ和Ⅳ型粘液细胞,而幽门胃中只有Ⅰ型粘液细胞,只含有中性粘多糖。幽门盲囊和肠道都含有中性粘多糖和酸性粘多糖,幽门盲囊以Ⅱ型粘液细胞最多,少量的Ⅲ型粘液细胞,前肠、中肠和后肠均有...
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| Published in | 南方水产科学 Vol. 11; no. 3; pp. 67 - 73 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国水产科学研究院南海水产研究所，农业部南海渔业资源开发利用重点实验室，广东 广州510300%中国水产科学研究院南海水产研究所，农业部南海渔业资源开发利用重点实验室，广东 广州510300
2015
上海海洋大学水产与生命学院，上海 201306 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0780 |
| DOI | 10.3969/j.issn.2095-0780.2015.03.011 |
Cover
| Abstract | 利用组织化学（AB-PAS）和免疫组织化学（SABC）的方法分别对斜带石斑鱼（Epinephelus coioides♀）×鞍带石斑鱼（E.lanceolatus♂）杂交子代（青龙斑）幼鱼消化道的粘液细胞和胃泌素（gastrin,Gas）分泌细胞的分布进行系统的研究。青龙斑幼鱼食道中有Ⅰ、Ⅱ、Ⅲ和Ⅳ型粘液细胞,含中性粘多糖和酸性粘多糖。贲门胃粘液细胞有Ⅰ、Ⅱ、Ⅲ种类型,胃体部有Ⅰ、Ⅲ、Ⅳ型粘液细胞,在胃腺的周围含有较多的Ⅰ和Ⅳ型粘液细胞,而幽门胃中只有Ⅰ型粘液细胞,只含有中性粘多糖。幽门盲囊和肠道都含有中性粘多糖和酸性粘多糖,幽门盲囊以Ⅱ型粘液细胞最多,少量的Ⅲ型粘液细胞,前肠、中肠和后肠均有4种类型的粘液细胞。肠道粘液细胞数量为中肠〉后肠〉前肠。利用免疫学的方法研究青龙斑幼鱼消化道Gas细胞的分布,表明幼鱼的整个肠道和幽门盲囊均有Gas细胞的存在,食道和胃中未发现Gas免疫阳性细胞。 |
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| AbstractList | S917.4; 利用组织化学（AB-PAS）和免疫组织化学（SABC）的方法分别对斜带石斑鱼（Epinephelus coioides ♀）×鞍带石斑鱼（E. lanceolatus ♂）杂交子代（青龙斑）幼鱼消化道的粘液细胞和胃泌素（gastrin，Gas）分泌细胞的分布进行系统的研究。青龙斑幼鱼食道中有Ⅰ、Ⅱ、Ⅲ和Ⅳ型粘液细胞，含中性粘多糖和酸性粘多糖。贲门胃粘液细胞有Ⅰ、Ⅱ、Ⅲ种类型，胃体部有Ⅰ、Ⅲ、Ⅳ型粘液细胞，在胃腺的周围含有较多的Ⅰ和Ⅳ型粘液细胞，而幽门胃中只有Ⅰ型粘液细胞，只含有中性粘多糖。幽门盲囊和肠道都含有中性粘多糖和酸性粘多糖，幽门盲囊以Ⅱ型粘液细胞最多，少量的Ⅲ型粘液细胞，前肠、中肠和后肠均有4种类型的粘液细胞。肠道粘液细胞数量为中肠＞后肠＞前肠。利用免疫学的方法研究青龙斑幼鱼消化道 Gas 细胞的分布，表明幼鱼的整个肠道和幽门盲囊均有 Gas 细胞的存在，食道和胃中未发现 Gas 免疫阳性细胞。 利用组织化学（AB-PAS）和免疫组织化学（SABC）的方法分别对斜带石斑鱼（Epinephelus coioides♀）×鞍带石斑鱼（E.lanceolatus♂）杂交子代（青龙斑）幼鱼消化道的粘液细胞和胃泌素（gastrin,Gas）分泌细胞的分布进行系统的研究。青龙斑幼鱼食道中有Ⅰ、Ⅱ、Ⅲ和Ⅳ型粘液细胞,含中性粘多糖和酸性粘多糖。贲门胃粘液细胞有Ⅰ、Ⅱ、Ⅲ种类型,胃体部有Ⅰ、Ⅲ、Ⅳ型粘液细胞,在胃腺的周围含有较多的Ⅰ和Ⅳ型粘液细胞,而幽门胃中只有Ⅰ型粘液细胞,只含有中性粘多糖。幽门盲囊和肠道都含有中性粘多糖和酸性粘多糖,幽门盲囊以Ⅱ型粘液细胞最多,少量的Ⅲ型粘液细胞,前肠、中肠和后肠均有4种类型的粘液细胞。肠道粘液细胞数量为中肠〉后肠〉前肠。利用免疫学的方法研究青龙斑幼鱼消化道Gas细胞的分布,表明幼鱼的整个肠道和幽门盲囊均有Gas细胞的存在,食道和胃中未发现Gas免疫阳性细胞。 |
| Abstract_FL | We studied the distribution of mucous and gastrin cells of digestive tract in Qinglong grouper(Epinephelus coioides ♀ × E. lanceolatus ♂)juveniles by histochemistry(AB-PAS)and immunohistochemistry(SABC). Mucous cells in esophagus of juvenile were of TypeⅠ,TypeⅡ,TypeⅢ and TypeⅣ containing neutral and acidic mucoitin;mucous cells of TypeⅠ,TypeⅡ and Type Ⅲwere found in cardiac stomach;mucous cells of TypeⅠ,Type Ⅲ and Type Ⅳ were found in fundic stomach. Many mucous cells of TypeⅠ,and Type Ⅳ could be found around gastric gland,and only mucous cells of TypeⅠcontaining neutral mucointin was found in pyloric stomach. Pyloric caeca and intestines contained neutral and acidic mucointin. Mucous cells in the pyloric caeca were mostly of Type Ⅱ but less Type Ⅲ. Four types of mucous cells could all be observed in foregut,midgut and hindgut. The descending order of number of intestinal mucous cells was midgut > hindgut > foregut. Moreover,the study on the distribution of gastrin cells in the diges-tive tract by immunohistochemistry indicates that the gastrin cells were found in entire intestine and pyloric caeca but not in esophagus and stomach. |
| Author | 李加儿 吴水清 区又君 刘江华 温久福 王鹏飞 |
| AuthorAffiliation | 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东广州510300 上海海洋大学水产与生命学院,上海201306 |
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| Author_FL | WU Shuiqing LI Jiaer WANG Pengfei OU Youjun WEN Jiufu LIU Jianghua |
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| DocumentTitleAlternate | Study on mucous and gastrin cells of digestive tract in Qinglong grouper （Epinephelus coioides ♀x E. lanceolatus ♂ ） juveniles |
| DocumentTitle_FL | Study on mucous and gastrin cells of digestive tract in Qinglong grouPer(Epinephelus coioides♀ × E. lanceolatus♂)juveniles |
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| Keywords | 消化道 Epinephelus coioides 斜带石斑鱼 E. lanceolatus hybrid 杂交子代 gastrin cells 胃泌素细胞 digestive tract 粘液细胞 mucous cells 鞍带石斑鱼 |
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| Notes | LI Jia＇er , WU Shuiqing, OU Youjun , LIU Jianghua, WEN Jiufu , WANG Pengfei （ 1. Key Lab. of South China Sea Fishery Resources Exploitation ＆ Utilization, Ministry of Agriculture ; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Seienees, Guangzhou 510300, China; 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China） Epinephelus coioides ; E. lanceolatus ; hybrid ; digestive tract ; mucous cells ; gastrin cells 44-1683/S We studied the distribution of mucous and gastrin cells of digestive tract in Qinglong grouper （ Epinephelus coioides ♀ x E. lanceolatus ♂ ） juveniles by histochemistry （AB-PAS） and immunohistochemistry （SABC）. Mucous cells in esophagus of juvenile were of Type Ⅰ , Type Ⅱ , Type Ⅲ and Type Ⅳcontaining neutral and acidic mucoitin; mucous cells of Type Ⅰ , Type Ⅱand Type Ⅲ were found in cardiac stomach; mucous cells of Type Ⅰ , Type Ⅲ and Type IV were found in fundic stomach. Many mucous cells of Type Ⅰ , and Type Ⅳ could be found arou |
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| Publisher | 中国水产科学研究院南海水产研究所，农业部南海渔业资源开发利用重点实验室，广东 广州510300%中国水产科学研究院南海水产研究所，农业部南海渔业资源开发利用重点实验室，广东 广州510300 上海海洋大学水产与生命学院，上海 201306 |
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| Snippet | 利用组织化学（AB-PAS）和免疫组织化学（SABC）的方法分别对斜带石斑鱼（Epinephelus coioides♀）×鞍带石斑鱼（E.lanceolatus♂）杂交子代（青龙斑）幼鱼消化道的粘液细胞... S917.4; 利用组织化学（AB-PAS）和免疫组织化学（SABC）的方法分别对斜带石斑鱼（Epinephelus coioides ♀）×鞍带石斑鱼（E. lanceolatus ♂）杂交子代（青龙斑）幼鱼消化道... |
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| Title | 斜带石斑鱼（♀）×鞍带石斑鱼（♂）杂交子代幼鱼消化道粘液细胞和胃泌素细胞的研究 |
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