斜带石斑鱼(♀)×鞍带石斑鱼(♂)杂交子代幼鱼消化道粘液细胞和胃泌素细胞的研究

利用组织化学(AB-PAS)和免疫组织化学(SABC)的方法分别对斜带石斑鱼(Epinephelus coioides♀)×鞍带石斑鱼(E.lanceolatus♂)杂交子代(青龙斑)幼鱼消化道的粘液细胞和胃泌素(gastrin,Gas)分泌细胞的分布进行系统的研究。青龙斑幼鱼食道中有Ⅰ、Ⅱ、Ⅲ和Ⅳ型粘液细胞,含中性粘多糖和酸性粘多糖。贲门胃粘液细胞有Ⅰ、Ⅱ、Ⅲ种类型,胃体部有Ⅰ、Ⅲ、Ⅳ型粘液细胞,在胃腺的周围含有较多的Ⅰ和Ⅳ型粘液细胞,而幽门胃中只有Ⅰ型粘液细胞,只含有中性粘多糖。幽门盲囊和肠道都含有中性粘多糖和酸性粘多糖,幽门盲囊以Ⅱ型粘液细胞最多,少量的Ⅲ型粘液细胞,前肠、中肠和后肠均有...

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Published in南方水产科学 Vol. 11; no. 3; pp. 67 - 73
Main Author 李加儿 吴水清 区又君 刘江华 温久福 王鹏飞
Format Journal Article
LanguageChinese
Published 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东 广州510300%中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东 广州510300 2015
上海海洋大学水产与生命学院,上海 201306
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ISSN2095-0780
DOI10.3969/j.issn.2095-0780.2015.03.011

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Summary:利用组织化学(AB-PAS)和免疫组织化学(SABC)的方法分别对斜带石斑鱼(Epinephelus coioides♀)×鞍带石斑鱼(E.lanceolatus♂)杂交子代(青龙斑)幼鱼消化道的粘液细胞和胃泌素(gastrin,Gas)分泌细胞的分布进行系统的研究。青龙斑幼鱼食道中有Ⅰ、Ⅱ、Ⅲ和Ⅳ型粘液细胞,含中性粘多糖和酸性粘多糖。贲门胃粘液细胞有Ⅰ、Ⅱ、Ⅲ种类型,胃体部有Ⅰ、Ⅲ、Ⅳ型粘液细胞,在胃腺的周围含有较多的Ⅰ和Ⅳ型粘液细胞,而幽门胃中只有Ⅰ型粘液细胞,只含有中性粘多糖。幽门盲囊和肠道都含有中性粘多糖和酸性粘多糖,幽门盲囊以Ⅱ型粘液细胞最多,少量的Ⅲ型粘液细胞,前肠、中肠和后肠均有4种类型的粘液细胞。肠道粘液细胞数量为中肠〉后肠〉前肠。利用免疫学的方法研究青龙斑幼鱼消化道Gas细胞的分布,表明幼鱼的整个肠道和幽门盲囊均有Gas细胞的存在,食道和胃中未发现Gas免疫阳性细胞。
Bibliography:LI Jia'er , WU Shuiqing, OU Youjun , LIU Jianghua, WEN Jiufu , WANG Pengfei ( 1. Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture ; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Seienees, Guangzhou 510300, China; 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China)
Epinephelus coioides ; E. lanceolatus ; hybrid ; digestive tract ; mucous cells ; gastrin cells
44-1683/S
We studied the distribution of mucous and gastrin cells of digestive tract in Qinglong grouper ( Epinephelus coioides ♀ x E. lanceolatus ♂ ) juveniles by histochemistry (AB-PAS) and immunohistochemistry (SABC). Mucous cells in esophagus of juvenile were of Type Ⅰ , Type Ⅱ , Type Ⅲ and Type Ⅳcontaining neutral and acidic mucoitin; mucous cells of Type Ⅰ , Type Ⅱand Type Ⅲ were found in cardiac stomach; mucous cells of Type Ⅰ , Type Ⅲ and Type IV were found in fundic stomach. Many mucous cells of Type Ⅰ , and Type Ⅳ could be found arou
ISSN:2095-0780
DOI:10.3969/j.issn.2095-0780.2015.03.011