High-yield cycloamylose production from sweet potato starch using Pseudomonas isoamylase and Thermus aquaticus 4-α-glucanotransferase

• Published in: Food science and biotechnology Vol. 25; no. 5; pp. 1413 - 1419
• Main Authors: Chu, Sun, Hong, Jung Sun, Rho, Shin-Joung, Park, Jiyoung, Han, Sang-Ik, Kim, Young-Wan, Kim, Yong-Ro
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society of Food Science and Technology 01.10.2016; Springer Nature B.V; 한국식품과학회
• Subjects: 4-alpha-glucanotransferase; Chemistry; Chemistry and Materials Science; Cycloamylose; Degree of polymerization; Food Science; Isoamylase; Nutrition; Polymerization; Potatoes; Pseudomonas; Solanum tuberosum; Starch; sweet potato starch; Sweet potatoes; Thermus aquaticus; 식품과학; starch modification; enzymatic conversion; normal starch; transglycosylation; debranching enzyme
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-016-0220-6

An optimal reaction condition for producing cycloamylose (CA) from sweet potato starch was investigated using a combination of isoamylase (from Pseudomonas sp.) and 4-α-glucanotransferase (from Thermus aquaticus , TAαGT). Starch was debranched by isoamylase for 8 h and subsequently reacted with TAαGT for 12 h. The yield and purity of CA products were determined using HPSEC and MALDI-TOFMS, respectively. Consequently, the maximum yield was 48.56%, exhibiting the highest CA production efficiency ever reported from starch. The CA products showed a wide range of the degree of polymerization (DP) with the minimum DP of 5. CA was also produced by simultaneous treatment of isoamylase and TAαGT. The yield was 3.31%, and the final products were contaminated by multiple branched and linear molecules. This result suggests that a former reaction condition (the sequential addition of isoamylase and TAαGT) is preferable for producing CA from sweet potato starch.