小麦蚕豆间作对根际土壤氮转化微生物的影响

通过田间小区试验,采用磷脂脂肪酸（PLFA）、实时荧光定量PCR研究了小麦蚕豆间作不同生育期对根际土壤微生物群落的变化、氨氧化细菌（AOB）、氨氧化古菌（AOA）以及反硝化细菌中亚硝酸还原酶（nir K）、一氧化氮还原酶（nor B）和氧化亚氮还原酶（nos Z）基因拷贝数以及土壤酶活性和土壤硝态氮、铵态氮含量的影响。结果表明：与单作相比,小麦蚕豆间作显著提高了根际土壤中总的PLFAs生物量、细菌、真菌、放线菌和好氧菌的生物量。土壤样品中的amo A基因拷贝数在105106范围内AOB的amo A基因数量高于AOA。在不同的生育期,根际土壤中nir K的基因拷贝数都是间作高于单作;在拔节期,间...

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Published in	农业资源与环境学报 Vol. 33; no. 5; pp. 482 - 490
Main Author	唐艳芬续勇波郑毅雷宝坤
Format	Journal Article
Language	Chinese
Published	农业农村部环境保护科研监测所 10.09.2016 中国农业生态环境保护协会
Subjects	amo A基因反硝化基因磷脂脂肪酸（PLFA）荧光定量PCR 间作 amoA基因磷脂脂肪酸(PLFA) 间作反硝化基因荧光定量PCR
Online Access	Get full text
ISSN	2095-6819 2095-6819

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Summary:	通过田间小区试验,采用磷脂脂肪酸（PLFA）、实时荧光定量PCR研究了小麦蚕豆间作不同生育期对根际土壤微生物群落的变化、氨氧化细菌（AOB）、氨氧化古菌（AOA）以及反硝化细菌中亚硝酸还原酶（nir K）、一氧化氮还原酶（nor B）和氧化亚氮还原酶（nos Z）基因拷贝数以及土壤酶活性和土壤硝态氮、铵态氮含量的影响。结果表明：与单作相比,小麦蚕豆间作显著提高了根际土壤中总的PLFAs生物量、细菌、真菌、放线菌和好氧菌的生物量。土壤样品中的amo A基因拷贝数在105106范围内AOB的amo A基因数量高于AOA。在不同的生育期,根际土壤中nir K的基因拷贝数都是间作高于单作;在拔节期,间作蚕豆的nor B基因显著高于其他种植模式（P〈0.05）;在拔节期、抽穗期,nos Z基因均是间作显著高于单作（P〈0.05）,并随着生育期呈现降低的趋势。间作降低了根际土壤NO-3-N的含量,提高了NH＋4-N的含量（P〈0.05）。说明小麦蚕豆间作后改变了根际土壤的微环境,使得土壤微生物群落结构发生改变,这种改变在一定程度上能够对土壤氮素的有效保蓄和供应、同时防止氮素损失和污染起到积极作用,为间作增产提供了氮素营养保障。
Bibliography:	12-1437/S intercropping; phospholipid fatty acids（PLFA）; quantitative real-time PC R; amoA genes; denitrifying genes Soil microorganism is one of the key factors that affects soil ecological activity. It is an important symbol of soil health, and the soil nitrogen cycle is closely related to the microorganisms. The relationship between nitrogen and microorganisms under the intercropping is im- portant for the farmland ecosystem. In this paper, phospholipid fatty acids （PLFA） analysis was used to determine soil microbial communi- ties, e.g., biomasses of anaerobic bacteria, aerobic bacteria, bacteria, fungi and actinobacteria. The abundance of nitrifying genes （AOB, AOA） and three denitrifying genes（nirK, norB, nosZ） were measured using real-time quantitative polymerase chain reaction. The enzymes, nitrate and ammonium concentrations were measured using conventional methods. The results showed that along with the growth period, the TPLFAs （total phospholipid fatty acids） increased and the bacterias, fungus, acti
ISSN:	2095-6819 2095-6819