高效液相色谱法分析内生真菌草酸青霉的代谢产物黑麦酮酸A

建立了高效液相色谱法（HPLC）测定内生真菌草酸青霉发酵液中黑麦酮酸A的方法。采用AgilentXDB-C18色谱柱（250 mm×4.6 mm,5μm）,确定了最佳色谱条件：流动相为甲醇和水（70∶30,V/V）,DAD检测器,检测波长为320 nm,流速为0.8 mL/min,柱温为30℃,进样量20μL。结果表明,在0.4～22 mg/L范围内,相关系数r=0.9996。在加标水平为0.5,5.0和15.0 mg/L时,平均回收率为86.0%～106.7%;相对标准偏差RSD〈6%;检出限为0.08 mg/L,具有较好的精密度和准确度。本方法已对10批实际样品进行发酵提取和分析,RSD=...

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Published in分析化学 Vol. 41; no. 4; pp. 575 - 579
Main Author 王艳红 吴晓民 朱艳萍 杨信东 李月茹
Format Journal Article
LanguageChinese
Published 吉林农业大学农业部参茸产品质量监督检验测试中心,长春,130118%吉林农业大学附属医院,长春,130118%吉林农业大学农学院,长春,130118 2013
Subjects
代谢产物
内生真菌
高效液相色谱
黑麦酮酸A
内生真菌
高效液相色谱
代谢产物
黑麦酮酸A
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ISSN0253-3820
DOI10.3724/SP.J.1096.2013.21141

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Summary:建立了高效液相色谱法（HPLC）测定内生真菌草酸青霉发酵液中黑麦酮酸A的方法。采用AgilentXDB-C18色谱柱（250 mm×4.6 mm,5μm）,确定了最佳色谱条件：流动相为甲醇和水（70∶30,V/V）,DAD检测器,检测波长为320 nm,流速为0.8 mL/min,柱温为30℃,进样量20μL。结果表明,在0.4～22 mg/L范围内,相关系数r=0.9996。在加标水平为0.5,5.0和15.0 mg/L时,平均回收率为86.0%～106.7%;相对标准偏差RSD〈6%;检出限为0.08 mg/L,具有较好的精密度和准确度。本方法已对10批实际样品进行发酵提取和分析,RSD=4.4%,可应用于内生真菌发酵优化过程中代谢产物黑麦酮酸A的定量分析。
Bibliography:High performance liquid chromatography; Endophytic Fungus; Secondary metabolites; Secalonic acid A
22-1125/O6
The secondary metabolite secalonic acid A from endophytic fungus has strong antitumor anti-inflammatory and antibacterial activities,which shows good development prospects for the new drugs.A method was developed for the determination of secalonic acid A in the broth of endophytic fungus Penicillium oxalicum by high performance liquid chromatography.Agilent XDB-C18（250 mm × 4.6 mm,5 μm） and DAD detector were used for the determination of secalonic acid A under the conditions as follows： methanol and water（70 ∶ 30,V/V） as mobile phase with a flow rate of 0.8 mL/min,320 nm of detection wavelength,30 ℃ of column temperature,and 20 μL of sample volume.The linearity,the detection limit,the precision and recovery were analysed.The results showed a good linear relationships in the range of 0.4-22 mg/L with a correlation coefficient of 0.9996.The mean recoveries were in the range of 86.0%-106.7% at three spiked
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2013.21141