核酸酶催化放大传感体系的设计及其在铅离子比色检测中的应用

基于8-17 DNA酶的催化切割特性和类辣根过氧化物酶DNA酶的氧化还原反应催化特性,发展了一种新型核酸酶催化放大传感体系,并用于Pb2＋的比色检测.考察了K＋浓度,pH值及反应时间对检测体系的影响.ABTS吸光度变化与Pb2＋浓度在5.0～100 nmol/L范围内呈良好的线性关系,检出限为3.0 nmol/L.此外,因Pb2＋酶的特异性,本方法对Pb2＋具有良好的选择性....

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Published in分析化学 Vol. 41; no. 5; pp. 670 - 674
Main Author 高晓霞 贾玉华 杨金凤 李继山 杨荣华
Format Journal Article
LanguageChinese
Published 湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,长沙410082%湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,长沙410082 2013
中南大学湘雅医学院附属肿瘤医院,长沙410013
Subjects
DNA酶
催化放大
比色检测
生物传感
铅离子
比色检测
催化放大
生物传感
铅离子
DNA酶
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ISSN0253-3820
DOI10.3724/SP.J.1096.2013.20678

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Summary:基于8-17 DNA酶的催化切割特性和类辣根过氧化物酶DNA酶的氧化还原反应催化特性,发展了一种新型核酸酶催化放大传感体系,并用于Pb2＋的比色检测.考察了K＋浓度,pH值及反应时间对检测体系的影响.ABTS吸光度变化与Pb2＋浓度在5.0～100 nmol/L范围内呈良好的线性关系,检出限为3.0 nmol/L.此外,因Pb2＋酶的特异性,本方法对Pb2＋具有良好的选择性.
Bibliography:Based on the catalytic cleavage of 8- 17 DNA enzyme （DNAzyme） and the catalytic redox of horseradish peroxidase （HRP）-mimicking DNAzyme, a novel DNAzyme catalytic amplification biosensing platform has been proposed for the colorimetric detection of lead ions （ Pb2＋ ）. The effects of K＋ concentration, pH value and incubation time on the detection system were investigated. The system exhibited a dynamic response range for Pb2＋ from 5 to 100 nmol/L with a detection limit of 3 nmol/L. In addition, the selectivity of the sensor for Pb2＋ against other environmentally related metal ions was outstanding.
GAO Xiao-Xia , JIA Yu-HuaI , YANG Jin-Feng＊ 1,2, LI Ji-Shan ＊ 1, YANG Rong-Hua1 1（ State Key Laboratory for Chemo/Biosensing and Chemometrics , College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China） 2 （Department of Anesthesiology, Tumor Hospital, Xiangya School of of Medicine of Central South University, Changsha 410013, China）
Deoxyribonucleic acid enzyme ; Colorimetric detection ; C
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2013.20678